Yu:PLAAC

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m
m
Line 4: Line 4:
|+'''Solution recipe for PLAAC(Protease inhibitor cocktail for yeast)'''.
|+'''Solution recipe for PLAAC(Protease inhibitor cocktail for yeast)'''.
|-
|-
-
| 1:1:1:1 of lab stock=1000x
+
| 3ng/μL Pepstatin
|-
|-
-
| Can add PMSF(1mM final)
+
| 3ng/μL Leupeptin
|-
|-
-
| Final concentration =3ng/μL of Pepstatin, Leu, Apro, Chy, Antipain
+
| 3ng/μL Antipain
|-
|-
 +
| 3ng/μL Aprotinin
 +
|-
 +
| 3ng/μL Chymostatin
 +
|-
 +
|Dissolve in DMSO. Aliquot 100μL per tube and freeze at -20°C.
 +
|}
[[Category:Yu Solutions]]
[[Category:Yu Solutions]]

Revision as of 10:15, 1 September 2011

Home       Procedures       | Lab Members       | Publications       | Research Interests       | Internal Database       Image:UBLogo.jpg


Solution recipe for PLAAC(Protease inhibitor cocktail for yeast).
3ng/μL Pepstatin
3ng/μL Leupeptin
3ng/μL Antipain
3ng/μL Aprotinin
3ng/μL Chymostatin
Dissolve in DMSO. Aliquot 100μL per tube and freeze at -20°C.
Personal tools