Yu:PLAAC

PLAAC is a standard protease inhibitor cocktail for yeast work. Stock concentration is 1000X (add 1μL per 1mL of lysis buffer). Thaw at room temperature due to DMSO. Keep PLAAC-complete solutions chilled.

Solution recipe for PLAAC Stock.

3ng/μL Pepstatin

3ng/μL Leupeptin

3ng/μL Antipain

3ng/μL Aprotinin

3ng/μL Chymostatin

Dissolve in DMSO. Aliquot 100μL per tube and freeze at -20°C.