Yu:Procedures
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m (added link to e. coli transformation) |
Current revision (12:45, 23 January 2013) (view source) m (link) |
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{|width="100%" border="0" cellpadding="10" | {|width="100%" border="0" cellpadding="10" | ||
| - | |width="30%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"> | + | |width="30%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Solutions</font></div> |
*[[Yu:YEPD|YEPD]] | *[[Yu:YEPD|YEPD]] | ||
| + | *[[Yu:SOC|SOC]] | ||
*[[Yu:Dropout Media|Dropout Media]] | *[[Yu:Dropout Media|Dropout Media]] | ||
*[[Yu:Lysis#buffer|Lysis Buffer]] | *[[Yu:Lysis#buffer|Lysis Buffer]] | ||
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**[[Yu:PBS#PBST|PBST]] | **[[Yu:PBS#PBST|PBST]] | ||
**[[Yu:PBS#PBSMT|PBSMT]] | **[[Yu:PBS#PBSMT|PBSMT]] | ||
| - | *[[Yu:TAE|TAE]] | + | *[[Yu:TBS|TBS]] |
| - | *[[Yu:TBE|TBE]] | + | **[[Yu:TBS#TBS-TT|TBS/TT]] |
| + | *[[Yu:TAE|50x TAE]] | ||
| + | *[[Yu:TBE|10x TBE]] | ||
*[[Yu:LiOAc|LiOAc/TE]] | *[[Yu:LiOAc|LiOAc/TE]] | ||
*[[Yu:IPP|IPP150]] | *[[Yu:IPP|IPP150]] | ||
| Line 21: | Line 24: | ||
*[[Yu:CBB|Calmodulin-Binding Buffer]] | *[[Yu:CBB|Calmodulin-Binding Buffer]] | ||
*[[Yu:TES|TES]] | *[[Yu:TES|TES]] | ||
| + | *[[Yu:Super_SPO|Super SPO Media]] | ||
| + | *[[Yu:P Solution|P Solution]] | ||
|width="70%" valign="top" align="left"| | |width="70%" valign="top" align="left"| | ||
{|border="0" cellpadding="10" | {|border="0" cellpadding="10" | ||
| - | |width=" | + | |width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top">[[Yu:Western|<font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Western Blot</font>]]</div> |
*[[Yu:Lysis|Lysing Cells]] | *[[Yu:Lysis|Lysing Cells]] | ||
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*[[Yu:Western#Immunoblotting|Immunoblotting]] | *[[Yu:Western#Immunoblotting|Immunoblotting]] | ||
*[[Yu:Western#Developing|Developing]] | *[[Yu:Western#Developing|Developing]] | ||
| + | *[[Yu:Western#Stripping|Stripping]] | ||
| + | |width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Transformation</font></div> | ||
| - | + | *[[Yu:Transformation|Yeast LiOAc Transformation]] | |
| - | + | ||
| - | + | ||
*[[Yu:e_coli_transform|''E. coli'' Transformation]] | *[[Yu:e_coli_transform|''E. coli'' Transformation]] | ||
| + | *[[Yu:Competent_Cells|''E. coli'' Normal Competent Cells]] | ||
| + | *[[Yu:quick_miniprep|Quick E. coli plasmid mini prep]] | ||
| + | *[[Yu:Yeast_miniprep|Yeast Plasmid Miniprep]] | ||
| + | |||
| + | |width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Southern Blot</font></div> | ||
| + | *[[Yu:southern_sol|Solutions]] | ||
| + | *[[Yu:Southern_Blotting|Southern Blotting]] | ||
|- | |- | ||
| - | |width=" | + | |width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">CoIP</font></div> |
| + | *[[Yu:CoIP|TAP CoIP]] | ||
| - | |width=" | + | |width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">PCR</font></div> |
| + | |||
| + | *[[Yu:homemade_taq|Homemade Taq]] | ||
| + | *[[Yu:ChIP_PCR|ChIP PCR (qPCR)]] | ||
| + | |||
| + | |width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Spheroplast Preperations</font></div> | ||
| + | *[[Yu:sphero_sol|Solutions]] | ||
| + | *[[Yu:spheroplast|Spheroplast preperation]] | ||
| + | *[[Yu:nuclei|Nuclei Isolation]] | ||
|- | |- | ||
| - | |width=" | + | |width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">ChIP</font></div> |
| - | |width=" | + | *[[Yu:TBE_gels|8% TBE polyacrylamide gel]] |
| + | |||
| + | |width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Northern Blot</font></div> | ||
*[[Yu:RNA_extract|RNA extraction]] | *[[Yu:RNA_extract|RNA extraction]] | ||
*[[Yu:Agarose_Formaldehyde|Agarose-Formaldehyde gels]] | *[[Yu:Agarose_Formaldehyde|Agarose-Formaldehyde gels]] | ||
*[[Yu:High-resolution_gels|High-resolution gels]] | *[[Yu:High-resolution_gels|High-resolution gels]] | ||
| - | *[[Yu:Northern_solns|Solutions]] | + | *[[Yu:Northern_solns|Northern Solutions]] |
*[[Yu:PNK_label|Oligo End Labeling]] | *[[Yu:PNK_label|Oligo End Labeling]] | ||
| + | |||
| + | |width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Chromatin mapping</font></div> | ||
| + | *[[Yu:MNase_dig|MNase digestion]] | ||
| + | *[[Yu:probe_design|Probe Design]] | ||
| + | |- | ||
| + | |width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Cloning</font></div> | ||
| + | *[http://tools.invitrogen.com/content/sfs/manuals/topota_man.pdf TOPO TA Manual] | ||
| + | *[[Yu:Genomic_prep|Rapid Genomic Prep]] | ||
| + | |width="33%" valign="top" align="left"|<div style="padding: 5px; color: #ffffff; background-color: #00349A; width: 100%; height: 23px; text-align: center; vertical-align: top"><font face="trebuchet ms" style="color:#ffffff; font-weight: bold">Misc</font></div> | ||
| + | *[[Yu:Dialysis|Dialysis]] | ||
| + | *[[Yu:Bead_Cleaning|Glass Bead Cleaning]] | ||
|} | |} | ||
| + | |- | ||
|} | |} | ||
Current revision
Solutions
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