Yu:Procedures

Solutions YEPD SOC Dropout Media Lysis Buffer SDS-PAGE Buffer Transfer Buffer for Western Blot PBS PBST PBSMT TBS TBS/TT 50x TAE 10x TBE LiOAc/TE IPP150 IPP300 NP-40 Buffer TEV Cleavage Buffer Calmodulin-Binding Buffer TES Super SPO Media P Solution

Western Blot Lysing Cells SDS-PAGE Gel Western Transfer Immunoblotting Developing Stripping Transformation Yeast LiOAc Transformation E. coli Transformation E. coli Normal Competent Cells Quick E. coli plasmid mini prep Yeast Plasmid Miniprep Southern Blot Solutions Southern Blotting CoIP TAP CoIP PCR Homemade Taq ChIP PCR (qPCR) Spheroplast Preperations Solutions Spheroplast preperation Nuclei Isolation ChIP 8% TBE polyacrylamide gel Northern Blot RNA extraction Agarose-Formaldehyde gels High-resolution gels Northern Solutions Oligo End Labeling Chromatin mapping MNase digestion Probe Design Cloning TOPO TA Manual Rapid Genomic Prep Misc Dialysis Glass Bead Cleaning