Yu:RNAse: Difference between revisions
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(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for RNAse(10mg/ml)'''. |- |Per 50ml: |- |1. 50ml MiliQ water |- |2. 0.5g RNAseA |- |3. 0.5ml TRis HCl(1M stock, pH 7.5...) |
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| Line 14: | Line 14: | ||
|4.0.0438g NaCl(15mM final) | |4.0.0438g NaCl(15mM final) | ||
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|5. | |5. Mix, heat to boil for 10 mins, filter sterilize (0.2μL) | ||
|- | |- | ||
|6.Make 1 ml aliquots,store at -20<sup>0</sup>C | |6.Make 1 ml aliquots,store at -20<sup>0</sup>C | ||
Revision as of 13:48, 18 September 2009
| Per 50ml: |
| 1. 50ml MiliQ water |
| 2. 0.5g RNAseA |
| 3. 0.5ml TRis HCl(1M stock, pH 7.5)(10mM final) |
| 4.0.0438g NaCl(15mM final) |
| 5. Mix, heat to boil for 10 mins, filter sterilize (0.2μL) |
| 6.Make 1 ml aliquots,store at -200C |
