Yu:RNAse

From OpenWetWare
Revision as of 13:47, 18 September 2009 by Christopher Jackson (talk | contribs) (New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for RNAse(10mg/ml)'''. |- |Per 50ml: |- |1. 50ml MiliQ water |- |2. 0.5g RNAseA |- |3. 0.5ml TRis HCl(1M stock, pH 7.5...)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)
Jump to navigationJump to search

Home       Procedures       | Lab Members       | Publications       | Research Interests       | Internal Database      


Solution recipe for RNAse(10mg/ml).
Per 50ml:
1. 50ml MiliQ water
2. 0.5g RNAseA
3. 0.5ml TRis HCl(1M stock, pH 7.5)(10mM final)
4.0.0438g NaCl(15mM final)
5. Mis, heat to boil for 10 mins, filter sterilize (0.2μL)
6.Make 1 ml aliquots,store at -200C