Yu:TBS

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|0.25% Tween-20
|0.25% Tween-20
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|-
-
|5. Add 10mL Triton-X
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|5. Add 10mL Triton-X100
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|1% Triton-X
+
|1% Triton-X100
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|-
|6. Stir until dissolved
|6. Stir until dissolved

Revision as of 20:07, 9 May 2010

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Solution recipe for 1L 5x Tris-Buffered Saline. Dilute to 1x for use.
1. Measure 700mL millipore water into a 2L plastic beaker. 5x
2. Weigh and add 43.83g NaCl, or add 150mL 5M NaCl 750mM NaCl
3. Add 50mL 1M TRIS-HCl pH 7.5 50mM TRIS pH 7.5
4. Stir until dissolved
5. Bring volume to 1L with millipore water in a graduated cylinder
6. Dilute from 5x to 1x (1:5) for use
Solution recipe for 1L 5x Tris-Buffered Saline / Tween-Triton. Dilute to 1x for use.
1. Measure 700mL millipore water into a 2L plastic beaker. 5x
2. Weigh and add 146.1g NaCl, or add 500mL 5M NaCl 2.5M NaCl
3. Add 100mL 1M TRIS-HCl pH 7.5 100mM TRIS pH 7.5
4. Add 2.5mL Tween-20 0.25% Tween-20
5. Add 10mL Triton-X100 1% Triton-X100
6. Stir until dissolved
7. Bring volume to 1L with millipore water in a graduated cylinder
8. Dilute from 5x to 1x (1:5) for use
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