Yu:TEV Cleavage Buffer: Difference between revisions
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{{Template:Yu}} | {{Template:Yu}} | ||
{| border="0" cellpadding="10" | {| border="0" cellpadding="10" width="100%" | ||
|+'''Solution recipe for | |+'''Solution recipe for 500 mL TEV Cleavage Buffer.''' | ||
|width=" | |width="66%" valign="top"|1. Measure 100 mL millipore H<sub>2</sub>O into a 500mL beaker | ||
|width=" | |width="33%" valign="top"| | ||
|- | |- | ||
|2. Add | |2. Add 12.5 mL of 1M TRIS-HCl, pH 8.0 | ||
| | |25 mM TRIS-HCl | ||
|- | |- | ||
|3. Add | |3. Add 15 mL of 5M NaCl | ||
| | |150 mM NaCl | ||
|- | |- | ||
|4. Add | |4. Add 500 μL of 0.5M EDTA | ||
| | |0.5 mM EDTA | ||
|- | |- | ||
|5. | |5. Add 5 mL 10% NP-40 | ||
| | |0.1% NP-40 | ||
|- | |- | ||
|6. Immediately prior to use, complete with 1μL of | |5. Bring volume to 500L with millipore H<sub>2</sub>O in a graduated cylinder | ||
| | | | ||
|- | |||
|6. Immediately prior to use, complete with 1μL of 1M DTT per mL TEV CB | |||
|1 mM DTT | |||
|} | |} | ||
[[Category:Yu Solutions]] | |||
Latest revision as of 11:58, 24 December 2009
| 1. Measure 100 mL millipore H2O into a 500mL beaker | |
| 2. Add 12.5 mL of 1M TRIS-HCl, pH 8.0 | 25 mM TRIS-HCl |
| 3. Add 15 mL of 5M NaCl | 150 mM NaCl |
| 4. Add 500 μL of 0.5M EDTA | 0.5 mM EDTA |
| 5. Add 5 mL 10% NP-40 | 0.1% NP-40 |
| 5. Bring volume to 500L with millipore H2O in a graduated cylinder | |
| 6. Immediately prior to use, complete with 1μL of 1M DTT per mL TEV CB | 1 mM DTT |
