Yu:TEV Cleavage Buffer: Difference between revisions
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{| border="0" cellpadding="10" | {| border="0" cellpadding="10" width="100%" | ||
|+'''Solution recipe for | |+'''Solution recipe for 1L TEV Cleavage Buffer.''' | ||
|width=" | |width="66%" valign="top"|1. Measure 500 mL millipore H<sub>2</sub>O into a 1L beaker | ||
|width=" | |width="33%" valign="top"| | ||
|- | |- | ||
|2. Add | |2. Add 50 mL of 1M TRIS-HCl, pH 8.0 | ||
| | |50 mM TRIS-HCl | ||
|- | |- | ||
|3. Add | |3. Add 30mL of 5M NaCl | ||
| | |150 mM NaCl | ||
|- | |- | ||
|4. Add | |4. Add 2 mL of 0.5M EDTA | ||
| | |1 mM EDTA | ||
|- | |- | ||
|5. | |5. Add 100 μL 100% Triton X-100 | ||
| | |0.01% Triton X-100 | ||
|- | |- | ||
| | |5. Bring volume to 1L with millipore H<sub>2</sub>O in a graduated cylinder | ||
| | |Volume to 1L | ||
|- | |- | ||
| | |6. Immediately prior to use, complete with DTT | ||
|1 mM DTT | |||
| | |||
|} | |} | ||
[[Category:Yu Solutions]] | [[Category:Yu Solutions]] | ||
Revision as of 10:37, 26 September 2009
| 1. Measure 500 mL millipore H2O into a 1L beaker | |
| 2. Add 50 mL of 1M TRIS-HCl, pH 8.0 | 50 mM TRIS-HCl |
| 3. Add 30mL of 5M NaCl | 150 mM NaCl |
| 4. Add 2 mL of 0.5M EDTA | 1 mM EDTA |
| 5. Add 100 μL 100% Triton X-100 | 0.01% Triton X-100 |
| 5. Bring volume to 1L with millipore H2O in a graduated cylinder | Volume to 1L |
| 6. Immediately prior to use, complete with DTT | 1 mM DTT |
