Yu:Tris: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
mNo edit summary |
||
| Line 21: | Line 21: | ||
|need approx. 42 ml HCl | |need approx. 42 ml HCl | ||
|} | |} | ||
[[Category:Yu Solutions]] | |||
Latest revision as of 14:26, 22 September 2009
| 1. Dissolve 121.1g of Tris base in 800ml of H2O. |
| 2. Adjust the pH to the desired value by adding concentrated HCl. Allow the solution to cool down to RT before making final adjustments to he pH |
| 3. Adjust the volume to 1 liter with H2O. Dispense into aliquots and sterilize by autoclaving |
| 7.4 | need approx. 70ml HCl |
| 7.6 | need approx. 60ml HCl |
| 8.0 | need approx. 42 ml HCl |
