Yu:Tris: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 1M Tris( Various pH)'''. |1. Dissolve 121.1g of Tris base in 800ml of H<sub>2</sub>O. |- |2. Adjust the pH to the ...) |
(No difference)
|
Revision as of 10:24, 18 September 2009
| 1. Dissolve 121.1g of Tris base in 800ml of H2O. |
| 2. Adjust the pH to the desired value by adding concentrated HCl. Allow the solution to cool down to RT before making final adjustments to he pH |
| 3. Adjust the volume to 1 liter with H2O. Dispense into aliquots and sterilize by autoclaving |
