Yu:Tris
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1. Dissolve 121.1g of Tris base in 800ml of H2O. |
2. Adjust the pH to the desired value by adding concentrated HCl. Allow the solution to cool down to RT before making final adjustments to he pH |
3. Adjust the volume to 1 liter with H2O. Dispense into aliquots and sterilize by autoclaving |