Yu:Tris-HCl: Difference between revisions
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(New page: {{Template:Yu}} {| border="0" cellpadding="10" |+'''Solution recipe for 1.5 M Tris-HCl, pH 8.8'''. |1. Add 54.45 g of Tris base into 150ml distilled water. |- |2. . |- |3. Adjust he PpH t...) |
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|+'''Solution recipe for 1.5 M Tris-HCl, pH 8.8'''. | |+'''Solution recipe for 1.5 M Tris-HCl, pH 8.8'''. | ||
|1. Add 54.45 g of Tris base | |1. Add 54.45 g of Tris base. | ||
|- | |- | ||
|2. . | |2. Add 150ml of distilled water. | ||
|- | |- | ||
|3. Adjust | |3. Adjust to pH 8.8 with 1N HCl. Millipore water to 300ml. Store at 4<sup>0</sup>C | ||
|- | |- | ||
Revision as of 10:34, 18 September 2009
| 1. Add 54.45 g of Tris base. |
| 2. Add 150ml of distilled water. |
| 3. Adjust to pH 8.8 with 1N HCl. Millipore water to 300ml. Store at 40C |
