Yu:Western
From OpenWetWare
Western Blotting
SDS PAGE
1.Cast SDS-PAGE gel and assemble the gel apparatus. |
2. Add 10μL of desired sample and 5μL 3x Gel loading buffer to an eppendorf tube and boil for 5 minutes. Do this in separate tubes for each sample to be analyzed. After boiling, samples may be kept at room temperature until loaded |
3. Load 12μL to 15μL of sample into each lane. Load protein prestain marker, as well as BenchMark, if desired. Load any empty lanes with 1x Gel loading buffer |
4. Run gel until the loading buffer is just running off the bottom of the gel |