Yu:YEPD: Difference between revisions
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|+'''Solution recipe for 1L liquid YEPD. Double for 2L, or use a higher multiplier for more.''' | |+'''Solution recipe for 1L <span id="liquid">liquid YEPD</span>. Double for 2L, or use a higher multiplier for more.''' | ||
|width="80%" valign="top"|1. Measure 800mL millipore water into a 2L plastic beaker. | |width="80%" valign="top"|1. Measure 800mL millipore water into a 2L plastic beaker. | ||
|width="20%" valign="top"|800mL H<sub>2</sub>O | |width="20%" valign="top"|800mL H<sub>2</sub>O | ||
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|+'''Solution recipe for 1L YEPD plates. Double for 2L, or use a higher multiplier for more.''' | |+'''Solution recipe for 1L <span id="plates">YEPD plates</span>. Double for 2L, or use a higher multiplier for more.''' | ||
|width="80%" valign="top"|1. Measure 800mL millipore water into a 2L plastic beaker. | |width="80%" valign="top"|1. Measure 800mL millipore water into a 2L plastic beaker. | ||
|width="20%" valign="top"|800mL H<sub>2</sub>O | |width="20%" valign="top"|800mL H<sub>2</sub>O |
Revision as of 10:44, 7 June 2009
1. Measure 800mL millipore water into a 2L plastic beaker. | 800mL H2O |
2. Weigh 10g Yeast Extract and add it to the mixture | 10g Yeast Extract |
3. Weigh 20g Bacto Peptone and add it to the mixture | 20g Bacto Peptone |
4. Weigh 20g Glucose and add it to the mixture | 20g Glucose |
5. Stir until completely dissolved | |
6. Bring solution to 1L with millipore water in a graduated cylinder | Volume to 1L |
7. Transfer to 2L flask, removing stir bar, and cap for autoclave | |
8. Autoclave 40 minutes to sterilize |
1. Measure 800mL millipore water into a 2L plastic beaker. | 800mL H2O |
2. Weigh 10g Yeast Extract and add it to the mixture | 10g Yeast Extract |
3. Weigh 20g Bacto Peptone and add it to the mixture | 20g Bacto Peptone |
4. Weigh 20g Glucose and add it to the mixture | 20g Glucose |
5. Stir until completely dissolved | |
6. Bring solution to 1L with millipore water in a graduated cylinder | Volume to 1L |
7. Transfer to 2L flask, removing stir bar, and add 20g Bacto Agar | 20g Bacto Agar |
8. Autoclave 40 minutes to sterilize | |
9. Allow to cool to 55-60°C while stirring with a sterile stir bar; add any desired drugs | |
10. Pour approximately 25mL per plate | |
11. Allow plates to cool and solidify |