Yu:southern sol

Alkaline Denaturation Solution

Final Concentration	For 1 Liter	For 4 Liters
1.5 M NaCl	87.7.3 g	351 g
0.5 M NaOH	20 g	80 g
milliQ H2O	to 1 L	to 4 L

Neutralization Solution

Final Concentration	For 1 Liter	For 4 Liters
1.5 M NaCl	87.7 g	351 g
0.5 M Tris Base	60 g	240 g
Adjust pH to 7.5 with HCl
milliQ H2O	to 1 L	to 4 L

Pre-hybridization Buffer

Final Concentration	For 100 mL	For 250 mL
6x SSC	30 mL of 20x SSC	75 mL of 20x SSC
2.5x Denhardt's	5 mL of 50x Denhardt's	12.5 mL of 50x Denhardt's
0.5% SDS	2.5 mL of 20% SDS	6.25 mL of 20% SDS
milliQ H2O	to 100 mL	to 250 mL
Store at RT or 4°C
Warm up the solution prior to use to redissolve the SDS

Hybridization Buffer

Final Concentration	For 100 mL	For 250 mL
6x SSC	30 mL of 20x SSC	75 mL of 20x SSC
1x Denhardt's	2 mL of 50x Denhardt's	3 mL of 50x Denhardt's
0.1% SDS	0.5 mL of 20% SDS	1.25 mL of 20% SDS
milliQ H2O	to 100 mL	to 250 mL
Store at RT or 4°C
Warm up the solution prior to use to redissolve the SDS