# Yu:southern sol

(Difference between revisions)
 Revision as of 14:30, 13 September 2009 (view source) (Added solution recipes)← Previous diff Revision as of 14:30, 13 September 2009 (view source)m (formatted table widths)Next diff → Line 44: Line 44: {| border="1" cellpadding="10" cellspacing="0" {| border="1" cellpadding="10" cellspacing="0" |width="300px" valign="top"|'''Final Concentration''' |width="300px" valign="top"|'''Final Concentration''' - |width="100px" valign="top"|'''For 100 mL''' + |width="150px" valign="top"|'''For 100 mL''' - |width="100px" valign="top"|'''For 250 mL''' + |width="150px" valign="top"|'''For 250 mL''' |- |- |6x SSC |6x SSC Line 73: Line 73: {| border="1" cellpadding="10" cellspacing="0" {| border="1" cellpadding="10" cellspacing="0" |width="300px" valign="top"|'''Final Concentration''' |width="300px" valign="top"|'''Final Concentration''' - |width="100px" valign="top"|'''For 100 mL''' + |width="150px" valign="top"|'''For 100 mL''' - |width="100px" valign="top"|'''For 250 mL''' + |width="150px" valign="top"|'''For 250 mL''' |- |- |6x SSC |6x SSC

## Contents

### Alkaline Denaturation Solution

 Final Concentration For 1 Liter For 4 Liters 1.5 M NaCl 87.7.3 g 351 g 0.5 M NaOH 20 g 80 g milliQ H2O to 1 L to 4 L

### Neutralization Solution

 Final Concentration For 1 Liter For 4 Liters 1.5 M NaCl 87.7 g 351 g 0.5 M Tris Base 60 g 240 g Adjust pH to 7.5 with HCl milliQ H2O to 1 L to 4 L

### Pre-hybridization Buffer

 Final Concentration For 100 mL For 250 mL 6x SSC 30 mL of 20x SSC 75 mL of 20x SSC 2.5x Denhardt's 5 mL of 50x Denhardt's 12.5 mL of 50x Denhardt's 0.5% SDS 2.5 mL of 20% SDS 6.25 mL of 20% SDS milliQ H2O to 100 mL to 250 mL Store at RT or 4°C Warm up the solution prior to use to redissolve the SDS

### Hybridization Buffer

 Final Concentration For 100 mL For 250 mL 6x SSC 30 mL of 20x SSC 75 mL of 20x SSC 1x Denhardt's 2 mL of 50x Denhardt's 3 mL of 50x Denhardt's 0.1% SDS 0.5 mL of 20% SDS 1.25 mL of 20% SDS milliQ H2O to 100 mL to 250 mL Store at RT or 4°C Warm up the solution prior to use to redissolve the SDS