Yu:southern sol

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Contents

Alkaline Denaturation Solution

Final Concentration For 1 Liter For 4 Liters
1.5 M NaCl 87.7.3 g 351 g
0.5 M NaOH 20 g 80 g
milliQ H2O to 1 L to 4 L

Neutralization Solution

Final Concentration For 1 Liter For 4 Liters
1.5 M NaCl 87.7 g 351 g
0.5 M Tris Base 60 g 240 g
Adjust pH to 7.5 with HCl
milliQ H2O to 1 L to 4 L

Pre-hybridization Buffer

Final Concentration For 100 mL For 250 mL
6x SSC 30 mL of 20x SSC 75 mL of 20x SSC
2.5x Denhardt's 5 mL of 50x Denhardt's 12.5 mL of 50x Denhardt's
0.5% SDS 2.5 mL of 20% SDS 6.25 mL of 20% SDS
milliQ H2O to 100 mL to 250 mL
Store at RT or 4°C
Warm up the solution prior to use to redissolve the SDS


Hybridization Buffer

Final Concentration For 100 mL For 250 mL
6x SSC 30 mL of 20x SSC 75 mL of 20x SSC
1x Denhardt's 2 mL of 50x Denhardt's 3 mL of 50x Denhardt's
0.1% SDS 0.5 mL of 20% SDS 1.25 mL of 20% SDS
milliQ H2O to 100 mL to 250 mL
Store at RT or 4°C
Warm up the solution prior to use to redissolve the SDS
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