ZEG reporter mouse

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(links)
m
Line 26: Line 26:
end: 3'
end: 3'
-
=== Links ===
+
== Links ==
* Novak & colleagues 2000 Genesis at [http://www.ncbi.nlm.nih.gov/pubmed/11105057 Pubmed] [http://onlinelibrary.wiley.com/doi/10.1002/1526-968X(200011/12)28:3/4%3C147::AID-GENE90%3E3.0.CO;2-G/pdf PDF]
* Novak & colleagues 2000 Genesis at [http://www.ncbi.nlm.nih.gov/pubmed/11105057 Pubmed] [http://onlinelibrary.wiley.com/doi/10.1002/1526-968X(200011/12)28:3/4%3C147::AID-GENE90%3E3.0.CO;2-G/pdf PDF]
* Z/EG mouse line = Tg(ACTB-Bgeo/GFP).. at JAX [http://jaxmice.jax.org/strain/003920.html] [http://jaxmice.jax.org/strain/004178.html]
* Z/EG mouse line = Tg(ACTB-Bgeo/GFP).. at JAX [http://jaxmice.jax.org/strain/003920.html] [http://jaxmice.jax.org/strain/004178.html]

Revision as of 10:05, 13 September 2010

Reporter mouse strain which can be switches by Cre recombination from LacZ to eGFP expression (therefore ZEG).

Contents

Description of the line

Original publication

Z/EG, a double reporter mouse line that expresses enhanced green fluorescent protein upon Cre-mediated excision.
Genesis. 2000 Nov-Dec;28(3-4):147-55.
Novak A, Guo C, Yang W, Nagy A, Lobe CG.
Cancer Research Division, Sunnybrook and Women's College Health Science Centre, Toronto, Ontario, Canada.
PMID 11105057

Excerpt from the abstract: "..We have developed a novel mouse line that acts as a Cre reporter. The mice, designated Z/EG (lacZ/EGFP), express lacZ throughout embryonic development and adult stages. Cre excision, however, removes the lacZ gene, which activates expression of the second reporter, enhanced green fluorescent protein. We have found that the double-reporter Z/EG line is able to indicate the occurrence of Cre excision from early embryonic to adult lineages..."

Transgene

start: 5'

  • promoter: pCAGGS
  • loxP site for Cre site-specific recombination
  • LacZ (bacterial β-galactosidase)
  • 3x pA signal
  • loxP site
  • eGFP
  • pA

end: 3'

Links

  • Novak & colleagues 2000 Genesis at Pubmed PDF
  • Z/EG mouse line = Tg(ACTB-Bgeo/GFP).. at JAX [1] [2]
Personal tools