Revision as of 15:38, 20 September 2016

Purpose

Uploaded the “visit_1_S1_S9.txt” and “visit_1_S10_S15.txt” files into the nucleic

acid tool set of the Biology Workbench by saving to ThawSpace

sequences to be uploaded at a time

Generated a multiple sequence alignment and distance tree for 12 of these sequences (See Table 1)
Unrooted tree was created using Biology Workbench
- Selected sequences
- Selected "CLUSTALW" and then "Submit"
The clones from each subject clustered together (Figure 1) however subject 15's sequences were more spread out than others
Some subjects’ clones show more diversity than others. This is observable by tracing the length of lines connecting the sequences from the same subject (ex: S15 and S1) (Figure 1)
None of the subjects really clustered together however it is difficult to differentiate the lengths of the terminal branches due to the lack of a definitive evolutionary beginning
Observations of the tree:
The Unrooted Tree was saved and uploaded to this assignment (right)

I received help on this assignment from User:Shivum A Desai
While I received help on this page everything completed for this assignment was done by me and was not copied from anyone else

All class assignments:

All individual assignments:

All shared journals:

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 =Methods=
+*Uploaded the “visit_1_S1_S9.txt” and “visit_1_S10_S15.txt” files into the nucleic
+acid tool set of the Biology Workbench by saving to ThawSpace
+*Two data files were used because the Biology Workbench only allows up to 64
+sequences to be uploaded at a time
+*Generated a multiple sequence alignment and distance tree for 12 of these sequences (See Table 1)
+*Unrooted tree was created using Biology Workbench
+**Selected sequences
+**Selected "CLUSTALW" and then "Submit"
+*The clones from each subject clustered together (Figure 1) however subject 15's sequences were more spread out than others
+*Some subjects’ clones show more diversity than others. This is observable by tracing the length of lines connecting the sequences from the same subject (ex: S15 and S1) (Figure 1)
+* None of the subjects really clustered together however it is difficult to differentiate the lengths of the terminal branches due to the lack of a definitive evolutionary beginning
+*Observations of the tree:
+**
+**
+**
+*The Unrooted Tree was saved and uploaded to this assignment (right)
 =Results=