Zhu Lab:Publications

Review

1. J. G. Forte* and L. Zhu*. 2010, Apical recycling of the gastric parietal cell H-K-ATPase, Annual Review of Physiology, 72: 21-44

---

Patents

1. Recombinant MVA capable of expressing structural HCV antigens. Inventors: Sutter G, Erfle V, Wang Y, Li G, Zhu L, Staib C. United States Patent 20050019347; Kind Code: A1. http://v3.espacenet.com/publicationDetails/biblio?CC=EP&NR=1423525&KC=&FT=E

---

Peer reviewed publications

1. Lixin Zhu*, Susan S. Baker*,Chelsea Gill,Wensheng Liu,Razan Alkhouri,Robert D. Baker,Steven R. Gill,2012,Characterization of the gut microbiome in non-alcoholic steatohepatitis (NASH) patients: A connection between endogenous alcohol and NASH, Hepatology, ‘Accepted Article’, doi: 10.1002/hep.26093.

Highlighted by Nature Reviews Gastroenterology and Hepatology 9, 687 (December 2012)|doi:10.1038/nrgastro.2012.209

1. Raza Patel, Susan S. Baker*, Wensheng Liu, Sonal Desai, Razan Alkhouri, Rafal Kozielski, Lucy Mastrandrea, Adil Sarfraz, Weijing Cai, Helen Vlassara, Mulchand S. Patel, Robert D. Baker, Lixin Zhu*, 2012, Effect of Dietary Advanced Glycation End Products on Mouse Liver, PLoS ONE,7(4): e35143. doi:10.1371/journal.pone.0035143.
2. Lixin Zhu, 2011, Teaching the fundamentals of biological research with primary literature: learning from the discovery of the gastric proton pump. Bioscene: Journal of College Biology Teaching. 37(2):3-8.
3. Shi Xu, Maria Edman, Mubashera S. Kothawala, Guoyong Sun, Lilian Chiang, Austin Mircheff, Lixin Zhu, Curtis Okamoto and Sarah Hamm-Alvarez, 2011, A Rab11a-enriched subapical membrane compartment regulates a cytoskeleton-dependent transcytotic pathway in secretory epithelial cells of the lacrimal gland. Journal of Cell Science. 124:3503-3514.
4. Wensheng Liu, Susan S. Baker, Robert D. Baker, Norma J. Nowak, Lixin Zhu*, 2011, Upregulation of Hemoglobin Expression by Oxidative Stress in Hepatocytes and Its Implication in Nonalcoholic Steatohepatitis. PLoS ONE 6(9): e24363. doi:10.1371/ journal.pone.0024363
5. Wenjun He, Wensheng Liu, Catherine S. Chew, Susan S. Baker, Robert D. Baker, John G Forte, and Lixin Zhu*, 2011, Acid Secretion-Associated Translocation of KCNJ15 in Gastric Parietal Cells. Am J Physiol Gastrointest Liver Physiol 301(4):G591-G600.
6. Lixin Zhu*, Susan S. Baker, Wensheng Liu, Meng-Hua Tao, Raza Patel and Robert D. Baker*, 2011, Lipid in the livers of adolescents with nonalcoholic steatohepatitis: combined effects of pathways on steatosis. Metabolism 60(7):1001-1011.
7. Jo Suda, Lixin Zhu, Serhan Karvar, 2011, Phosphorylation of radixin regulates cell polarity and mrp-2 distribution in hepatocytes. Am J Physiol Cell Physiol 300(3):C416-C424.
8. Jo Suda, Lixin Zhu, Curtis T. Okamoto, Serhan, Karvar, 2011, Rab27b localizes to the tubulovesicle membranes of gastric parietal cells and regulates acid secretion, Gastroenterology 140(3):868-878.e2. Epub 2010 Oct 1.
9. Baker SS*, Baker RD, Liu W, Nowak NJ, Zhu L*; Increased hemoglobin expression in NASH livers; Gene Expression Omnibus; 2010 Oct;
10. Lixin Zhu, James Crothers, Jr., Rihong Zhou and John G. Forte, 2010, A Possible Mechanism for Ezrin to Establish Epithelial Cell Polarity. Am J Physiol Cell Physiol 299: C431-C443.
11. Susan S. Baker, Robert D. Baker, Wensheng Liu, Norma J. Nowak, and Lixin Zhu*, 2010, Role of alcohol metabolism in non-alcoholic steatohepatitis. PLoS ONE,5(3): e9570. doi:10.1371/journal.pone.0009570.
12. Baker SS*, Baker RD, Liu W, Nowak NJ, Zhu L*; Global gene expression of NASH patients; Gene Expression Omnibus; 2009 Aug;
13. Lixin Zhu, Jason Hatakeyama, Bing Zhang, Joy Makdisi, Cody Ender, and John G. Forte, 2009, Novel insights of the gastric gland organization revealed by chief cell specific expression of moesin. Am J Physiol Gastrointest Liver Physiol 296:G185-95.
14. Lixin Zhu, 2008, Teaching glycoprotein with a classical paper: knowledge and methods in the course of an exciting discovery ---- The story of discovering HK-ATPase-subunit. Biochemistry and Molecular Biology Education. 36:336-340.
15. Lixin Zhu, Jason Hatakeyama, Cheng Chen, Aditi Shastri, Kevin Poon and John G. Forte, 2008, Comparative study of ezrin phosphorylation among different tissues: more is good; too much is bad. Am J Physiol Cell Physiol 295:C192-202.
16. Lixin Zhu, Rihong Zhou, Shelley Mettler, Tim Wu, Aennes Abbas, Joseph Delaney and John G. Forte, 2007, High turnover of ezrin T567 phosphorylation: conformation, activity and cellular function. Am J Physiol Cell Physiol 293:C874-84.
17. Stephanie Tenhumberg, Bjoern Schuster, Lixin Zhu, Marina Kovaleva, Juergen Scheller, Karl-Josef Kallen and Stefan Rose-John, 2006, gp130 dimerization in the absence of ligand: Preformed cytokine receptor complexes, Biochemical and Biophysical Research Communications. 346:649-657.
18. Rihong Zhou, Lixin Zhu, Andrew Kodani, Paul Hauser, Xuebiao Yao, John G. Forte, 2005, Phosphorylation of ezrin on threonine 567 produces a change in secretory phenotype and repolarizes the gastric parietal cells, Journal of Cell Science. 118:4381-4391.
19. Serhan Karvar, Lixin Zhu, James Crothers Jr, Waylan Wong, Mustafa Turkoz, John G. Forte, 2005, Cellular localization and stimulation-associated distribution dynamics of syntaxin-1 and syntaxin-3 in gastric parietal cells, Traffic. 6:1-13.
20. Lixin Zhu, Yuechueng Liu, John G. Forte, 2005, Ezrin oligomers are the membrane-bound dormant form in gastric parietal cells, Am J Physiol Cell Physiol 288:C1242-C1254.
21. Jing Liu, Li-Xin Zhu, Yu-Ying Kong, Guang-Di Li, Yuan Wang, 2005, Purification and application of C-terminally truncated hepatitis C virus E1 proteins expressed in Escherichia coli, World Journal of Gastroenterology. 11: 503-507.
22. Li-Xin Zhu, Jing Liu, Ye Ye, You-Hua Xie, Guang-Di Li, Yuan Wang, 2004, A candidate DNA vaccine elicited HCV specific humoral and cellular immune responses, World Journal of Gastroenterology. 10:2488-2492.
23. Li-Xin Zhu, Jing Liu, You-Hua Xie, Yu-Ying Kong, Ye Ye, Chun-Lin Wang, Guang-Di Li, Yuan Wang, 2004, Expression of hepatitis C virus envelope protein 2 induced apoptosis in cultured mammalian cells, World Journal of Gastroenterology. 10:2972-2978.
24. ZHU Jun, WANG Chun-Lin, ZHU Li-Xin, KONG Yu-Ying, WANG Yuan, LI Guang-Di, 2002, DNA immunization with different recombinant HCV E2 expression plasmids, Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Acta Biochemica et Biophysica Sinica) 34:445-451.
25. WANG Chun-Lin, ZHU Li-Xin, LIU Jing, ZHANG Zu-Chuan, WANG Yuan, and LI Guang-Di, 2002, Expression and characterization of hepatitis C virus E2 glycoprotein fused to hepatitis B virus preS1(21-47) fragment in CHO cells, Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Acta Biochemica et Biophysica Sinica) 34:400-404.
26. Jing Liu, Yuying Kong, Lixin Zhu, Yuan Wang and Guangdi Li, 2002, High-level expression of the C-terminal hydrophobic region of HCV E2 protein ectodomain in E. coli, Virus genes. 25:5-13.
27. Li-Xin Zhu, Jing Liu, Ying-Chun Li, Yu-Ying Kong, Caroline Staib, Gerd Sutter, Yuan Wang, and Guang-Di Li, 2002, Full-length core sequence dependent complex-type glycosylation of hepatitis C virus E2 glycoprotein，World Journal of Gastroenterology. 8:499-504.
28. Jing Liu, Lixin Zhu, Xinxin Zhang, Min Lu, Yuying Kong, Yuan Wang, and Guangdi Li, 2001, Expression, purification, immunological characterization, and application of Escherichia coli-derived hepatitis C virus E2 proteins, Biotechnology and applied biochemistry, 34:109-119.
29. ZHU Li-Xin, Kong Yu-Ying, WANG Yuan, and LI Guang-Di, 2001, Effect of downstream sequence on the cleavage of envelop protein 1 signal sequence in hepatitis C virus,Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Acta Biochemica et Biophysica Sinica). 33：682-686.
30. ZHU Li-Xin, XIE You-Hua, LI Guang-Di, and WANG Yuan, 2001, High frequency of homologous recombination in the genome of modified vaccinia virus Ankara strain (MVA), Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Acta Biochemica et Biophysica Sinica). 33:497-503.
31. YANG Li, ZHU Li-Xin, WANG Yuan, and LI Guang-Di. 1999, Fusion Expression, Immunogenicity and Applications of C-terminally Truncated HCV Core Proteins. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Acta Biochemica et Biophysica Sinica) 31:61-66.

---

Abstracts (Presentations at International Conferences)

1. W. Liu, S.S. Baker, R.D. Baker, L. Zhu*, May 2012, Inhibition of lysosomal enzyme activities by proton pump inhibitors, Digestive Diseases Week 2012, Abstract# 1298923, Oral presentation. San Diego, CA.
2. R.A. Patel, S.S. Baker, W. Liu, S. Desai, R. Alkhouri, R. Kozielski, L. Mastrandrea, A. Sarfraz, W. Cai, H. Vlassara, M.S. Patel, R.D. Baker, L. Zhu*, April 2012, Effect of dietary advanced glycation end products on mouse liver, Experimental Biology 2012, abstract# 3257, Oral presentation. San Diego, CA.
3. S. Desai, S. Baker, W. Liu, R. Kozielski, R. Browne, R. Patel, R. Baker, L. Zhu*, October 20, 2011, Impact of paraoxonase 1(PON1) in pediatric non-alcoholic steatohepatitis. North American Society for Pediatric Gastroenterology, Hepatology and Nutrition (NASPGHAN) National meeting, Orlando, FL.
4. R.H. Alkhouri, S.S. Baker, H. Hashmi, R.D. Baker, W. Liu, D. Gelfond, L. Zhu*, October 20, 2011, Paraoxonase gene expression in patients with inflammatory bowel disease, NASPGHAN National meeting Orlando, FL.
5. H. Hashmi, R.D. Baker, R. Alkhouri, L. Zhu*, October 20, 2011, RUNX3 expression in inflammatory bowel disease, NASPGHAN National meeting, Orlando, FL.
6. S. Desai, S. Baker, W. Liu, R. Kozielski, R. Browne, R. Patel, R. Baker, L. Zhu*, May 2011, Elevated Paraoxonase 1 (PON1) Activity in Pediatric Non-Alcoholic Steatohepatitis (NASH) Livers, University of Buffalo Scholarly Exchange Day, Buffalo, NY.
7. S. S. Desai, S. Baker, W. Liu, R. D. Baker, L. Zhu*, Elevated paraoxonase 1(PON1) activity in pediatric non-alcoholic steatohepatitis (NASH) livers, Digestive Disease Week 2011, Chicago, IL. Board No. Mo2026.
8. W. He, W. Liu., C.S. Chew, S. S. Baker, R. D. Baker, J. G. Forte and L. Zhu*, December 2010, Acid secretion associated translocation of KCNJ15 in gastric parietal cells, 50th Annual meeting of The American Society for Cell Biology, Philadelphia, PA, Program No./Board No.: 1204/B434.
9. W. Liu, S. S. Baker, R. D. Baker, N. J. Nowak, L. Zhu*, December 2010, Hemoglobin is a possible antioxidant in hepatocytes, 50th Annual meeting of The American Society for Cell Biology, Philadelphia, PA, Program No./Board No.: 725/B1135.
10. J. Suda, L. Zhu, S. Karvar S, May 2010, Distribution Dynamics of Radixin and NHERF-1 on Regulation of MRP-2 Trafficking in Hepatocytes. Digestive Disease Week 2010, Gastroenterology Vol. 138, Issue 5, Supplement 1, Page S-782
11. J. Suda, L. Zhu, S. Karvar S, 2009, Intracellular localization of Radixin and NHERF-1 on regulation of mrp-2 trafficking in hepatocytes. The Liver Meeting-AASLD’s 60st annual meeting, Abstract #: 659, Hepatology 50: 615A.
12. J. Suda, L. Zhu, S. Karvar, May 2009, Intracellular localization and functional importance of Rab27b in gastric parietal cells, Digestive Disease Week 2009, Chicago, IL. Abstract #: S1626, Gastroenterol 136: A174.
13. L. Zhu, B. Zhang, J. Makdisi, J. Hatakeyama, C. Ender, J. Forte, December 2008, Chief cell specific expression of moesin in gastric glands, 48th Annual meeting of The American Society for Cell Biology, San Francisco, CA. abstract #346, board #B290.
14. S. Karvar, Suda J., L. Zhu, 2008, Cellular localization and stimulation dependent distribution of Munc-18b in gastric parietal cells. Digestive Disease Week 2008, Gastroenterol 134: A573.
15. L. Zhu, K. Poon, B. Zhang, M. Starr, C. Ender, J. Forte, December 2008, Phosphoisoforms of ezrin in gastric glands and proximal tubules, 48^th Annual meeting of The American Society for Cell Biology, San Francisco, CA. abstract #347, board #B291.
16. L. Zhu, J. Hatakeyama, A. Shastri, C. Chen, K. Poon, and J.G. Forte, April 2008, Different cell surface structures have different levels of ezrin phosphorylation, Experimental Biology Annual meeting 2008, San Diego, California, Oral presentation and poster presentation, abstract #5262
17. L. Zhu, R. Zhou, S. Mettler, T. Wu, A. Abbas, and J. G. Forte, April 2007, Reversible phosphorylation of ezrin T567: conformation, activity and physiology, Experimental Biology Annual meeting 2007, Washington D.C. FASEB J. 2007 21:760.5
18. L. Zhu, A. Abbas, T. Wu, S. Patel, S. Karvar, C.C. Yun, and J.G. Forte, December 2006, Phosphorylation of NHERF1/EBP50 in parietal cells, 46^th Annual meeting of The American Society for Cell Biology, San Diego, CA. pg 58, abstract #416, board #B373.
19. S. Karvar, L. Zhu, J. M. Crothers, and J. G. Forte, May 2006, Identification of Rab27b in gastric parietal cells, 107^th annual meeting of the American gastroenterological association, Los Angeles, CA. Gastroenterology. 130(4) (Suppl. 2): A-408. Abstract#: M2075.
20. L. Zhu, S. Karvar, J. Trinidad, V. Lao, J. Crothers, A. Burlingame, and J. Forte, August 2005, Modification of ATP synthase alpha is associated with stimulated acid secretion by gastric glands, Seventh international symposium on mass spectrometry in the health and life sciences, San Francisco, CA. Molecular & cellular proteomics. 4(8)(Suppl. 2): S472.
21. S. Karvar, L. Zhu, W. Wong, J. M. Crothers, and J. G. Forte, May 2005, Differencial distribution dynamics of syntaxin-1 and syntaxin-3 in gastric parietal cells, 106^th annual meeting of the American gastroenterological association, Chicago, IL. Gastroenterology. 128(4)(Suppl. 2):A-589. Abstract#: W1319.
22. R. Zhou, A. Kodani, V. Li, L. Zhu, J. Forte, December 2004, Phosphorylation of threonine-567 is not a functionally important site for apical membrane recruitment in parietal cells, 44^th Annual meeting of The American Society for Cell Biology, Washington D.C. Molecular biology of the cell. 15:171a, abstract # 950.
23. L. Zhu, Y. Liu, J. G. Forte, December 2004, Ezrin oligomers are the membrane-bound dormant form in gastric parietal cells, 44^th Annual meeting of The American Society for Cell Biology, Washington D.C. Molecular biology of the cell. 15:171a, abstract # 948.

---

Teaching publication

1. Lixin Zhu, 2011, Teaching the fundamentals of biological research with primary literature: learning from the discovery of the gastric proton pump. Bioscene: Journal of College Biology Teaching. 37(2):3-8.
2. Lixin Zhu, 2008, Teaching glycoprotein with a classical paper: knowledge and methods in the course of an exciting discovery ---- The story of discovering HK-ATPase-subunit. Biochemistry and Molecular Biology Education. 36:336-340.

---

Teaching Course

1. Discovery Seminar Program-Fall 2010, 309706,UB Discovery Seminar Program
2. Discovery Seminar Program-Spring 2011, 024542,UB Discovery Seminar Program
3. Discovery Seminar Program-Fall 2011, 25681,UB Discovery Seminar Program
4. Discovery Seminar Program-Spring 2012, 17957,UB Discovery Seminar Program
5. Discovery Seminar Program-Fall 2012, 12166,UB Discovery Seminar Program
6. Discovery Seminar Program-Spring 2013, 18934,UB Discovery Seminar Program

Current Research on Gastric Acid Secretion: the Niagara Falls in the Stomach

The seminar will center on the beauty of the acid secretion by the gastric parietal cell, which is similarly amazing as the Niagara Falls. There is a huge fall of proton concentration (a difference of 1 million fold) between the plasma membrane of parietal cells. Will cover the history of gastric acid research from 1800s to today, the major mechanisms for the regulation of acid secretion, how the most important discoveries in this field were made, what remains unknown. Students will review parts of gastric acid research, and participate in the ongoing research in the lab.

Seminar Highlights

---

1. The instructor (Dr. Lixin Zhu) will help you appreciate a natural wonder, similarly amazing as the Niagara Falls.

2. A chance to know how cutting-edge biology research is done in my lab.

3. Get to know some interesting stories about the legendary scientists in this field.

4. A taste of the modern techniques in a biology lab.

5. Why eating raw meat is no longer an issue to me after I studied the stomach?

6. Dr. Lixin Zhu was one of postdoc student of Dr. John G. Forte (University of California, Berkeley).

Dr. Forte discovered the alpha chain of proton pump (H,K-ATPase) in 1967, discovered the beta chain of the pump in 1990. More

beautifully, he and his wife discovered the elegant mechanism for the regulation of acid secretion in 1977. He made many other

important discoveries in the field.

7. Dr. Lixin Zhu discovered a major mechanism for the regulation of the cytoskeleton in the acid secreting parietal cell. He is looking into the mystery of membrane trafficking and membrane fusion in this cell.

8. 2010-2011 Discovery Seminar Course Award, University at Buffalo

---