Zhu Lab:Publications

Patents (one invention applied in different countries)

1. Recombinant MVA capable of expressing structural HCV antigens. Inventors: Sutter G, Erfle V, Wang Y, Li G, Zhu L, Staib C. United States Patent 20050019347; Kind Code: A1. http://www.freepatentsonline.com/20050019347.html
2. Recomninantes MVA mit der Faehigheit zur Expression von HCV Struktur-Antigenen. German patent.

http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=EP1423525

Peer reviewed publications (Cited 81 times in total by April, 2008)

1. Lixin Zhu, Jason Hatakeyama, Bing Zhang, Joy Makdisi, Cody Ender, and John G. Forte, 2009, Novel insights of the gastric gland organization revealed by chief cell specific expression of moesin. Am J Physiol Gastrointest Liver Physiol. 296:G185-95.
2. Lixin Zhu, 2008, Teaching glycoprotein with a classical paper: knowledge and methods in the course of an exciting discovery ---- The story of discovering HK-ATPase -subunit. Biochemistry and Molecular Biology Education. 36:336-340.
3. Lixin Zhu, Jason Hatakeyama, Cheng Chen, Aditi Shastri, Kevin Poon and John G. Forte, 2008, Comparative study of ezrin phosphorylation among different tissues: more is good; too much is bad. Am J Physiol Cell Physiol. 295:C192-202.
4. Lixin Zhu, Rihong Zhou, Shelley Mettler, Tim Wu, Aennes Abbas, Joseph Delaney and John G. Forte, 2007, High turnover of ezrin T567 phosphorylation: conformation, activity and cellular function. Am J Physiol Cell Physiol. 293:C874-84.
5. Stephanie Tenhumberg, Bjoern Schuster, Lixin Zhu, Marina Kovaleva, Juergen Scheller, Karl-Josef Kallen and Stefan Rose-John, 2006, gp130 dimerization in the absence of ligand: Preformed cytokine receptor complexes, Biochemical and Biophysical Research Communications. 346:649-657.
6. Rihong Zhou, Lixin Zhu, Andrew Kodani, Paul Hauser, Xuebiao Yao, John G. Forte, 2005, Phosphorylation of ezrin on threonine 567 produces a change in secretory phenotype and repolarizes the gastric parietal cells, Journal of Cell Science. 118:4381-4391.
7. Serhan Karvar, Lixin Zhu, James Crothers Jr, Waylan Wong, Mustafa Turkoz, John G. Forte, 2005, Cellular localization and stimulation-associated distribution dynamics of syntaxin-1 and syntaxin-3 in gastric parietal cells, Traffic. 6:1-13.
8. Lixin Zhu, Yuechueng Liu, John G. Forte, 2005, Ezrin oligomers are the membrane-bound dormant form in gastric parietal cells, Am J Physiol Cell Physiol. 288:C1242-C1254.
9. Jing Liu, Li-Xin Zhu, Yu-Ying Kong, Guang-Di Li, Yuan Wang, 2005, Purification and application of C-terminally truncated hepatitis C virus E1 proteins expressed in Escherichia coli, World Journal of Gastroenterology. 11: 503-507.
10. Li-Xin Zhu, Jing Liu, Ye Ye, You-Hua Xie, Guang-Di Li, Yuan Wang, 2004, A candidate DNA vaccine elicited HCV specific humoral and cellular immune responses, World Journal of Gastroenterology. 10:2488-2492.
11. Li-Xin Zhu, Jing Liu, You-Hua Xie, Yu-Ying Kong, Ye Ye, Chun-Lin Wang, Guang-Di Li, Yuan Wang, 2004, Expression of hepatitis C virus envelope protein 2 induced apoptosis in cultured mammalian cells, World Journal of Gastroenterology. 10:2972-2978.
12. ZHU Jun, WANG Chun-Lin, ZHU Li-Xin, KONG Yu-Ying, WANG Yuan, LI Guang-Di, 2002, DNA immunization with different recombinant HCV E2 expression plasmids, Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Acta Biochemica et Biophysica Sinica). 34:445-451.
13. WANG Chun-Lin, ZHU Li-Xin, LIU Jing, ZHANG Zu-Chuan, WANG Yuan, and LI Guang-Di, 2002, Expression and characterization of hepatitis C virus E2 glycoprotein fused to hepatitis B virus preS1(21-47) fragment in CHO cells, Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Acta Biochemica et Biophysica Sinica). 34:400-404.
14. Jing Liu, Yuying Kong, Lixin Zhu, Yuan Wang and Guangdi Li, 2002, High-level expression of the C-terminal hydrophobic region of HCV E2 protein ectodomain in E. coli, Virus genes. 25:5-13.
15. Li-Xin Zhu, Jing Liu, Ying-Chun Li, Yu-Ying Kong, Caroline Staib, Gerd Sutter, Yuan Wang, and Guang-Di Li, 2002, Full-length core sequence dependent complex-type glycosylation of hepatitis C virus E2 glycoprotein，World Journal of Gastroenterology. 8:499-504.
16. Jing Liu, Lixin Zhu, Xinxin Zhang, Min Lu, Yuying Kong, Yuan Wang, and Guangdi Li, 2001, Expression, purification, immunological characterization, and application of Escherichia coli-derived hepatitis C virus E2 proteins, Biotechnology and applied biochemistry, 34:109-119.
17. ZHU Li-Xin, Kong Yu-Ying, WANG Yuan, and LI Guang-Di, 2001, Effect of downstream sequence on the cleavage of envelop protein 1 signal sequence in hepatitis C virus, Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Acta Biochemica et Biophysica Sinica). 33：682-686.
18. ZHU Li-Xin, XIE You-Hua, LI Guang-Di, and WANG Yuan, 2001, High frequency of homologous recombination in the genome of modified vaccinia virus Ankara strain (MVA), Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Acta Biochemica et Biophysica Sinica). 33:497-503.
19. YANG Li, ZHU Li-Xin, WANG Yuan, and LI Guang-Di. 1999, Fusion Expression, Immunogenicity and Applications of C-terminally Truncated HCV Core Proteins. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Acta Biochemica et Biophysica Sinica). 31: 61-66.

Review

1.J. G. Forte and L. Zhu. 2010, Apical recycling of the gastric parietal cell H-K-ATPase, Annual Review of Physiology, 72: 21-44

Abstracts (Presentations at International Conferences)

1. J. Suda, L. Zhu, S. Karvar, May 2009, Intracellular localization and functional importance of Rab27b in gastric parietal cells, Digestive Disease Week 2009, Chicago, IL.
2. L. Zhu, B. Zhang, J. Makdisi, J. Hatakeyama, C. Ender, J. Forte, December 2008, Chief cell specific expression of moesin in gastric glands, 48^th Annual meeting of The American Society for Cell Biology, San Francisco, CA. abstract #346, board #B290.
3. L. Zhu, K. Poon, B. Zhang, M. Starr, C. Ender, J. Forte, December 2008, Phosphoisoforms of ezrin in gastric glands and proximal tubules, 48^th Annual meeting of The American Society for Cell Biology, San Francisco, CA. abstract #347, board #B291.
4. L. Zhu, J. Hatakeyama, A. Shastri, C. Chen, K. Poon, and J.G. Forte, April 2008, Different cell surface structures have different levels of ezrin phosphorylation, Experimental Biology Annual meeting 2008, San Diego, California, Oral presentation and poster presentation, abstract #5262
5. L. Zhu, R. Zhou, S. Mettler, T. Wu, A. Abbas, and J. G. Forte, April 2007, Reversible phosphorylation of ezrin T567: conformation, activity and physiology, Experimental Biology Annual meeting 2007, Washington D.C. FASEB J. 2007 21:760.5
6. L. Zhu, A. Abbas, T. Wu, S. Patel, S. Karvar, C.C. Yun, and J.G. Forte, December 2006, Phosphorylation of NHERF1/EBP50 in parietal cells, 46^th Annual meeting of The American Society for Cell Biology, San Diego, CA. pg 58, abstract #416, board #B373.
7. S. Karvar, L. Zhu, J. M. Crothers, and J. G. Forte, May 2006, Identification of Rab27b in gastric parietal cells, 107^th annual meeting of the American gastroenterological association, Los Angeles, CA. Gastroenterology. 130(4) (Suppl. 2): A-408. Abstract#: M2075.
8. L. Zhu, S. Karvar, J. Trinidad, V. Lao, J. Crothers, A. Burlingame, and J. Forte, August 2005, Modification of ATP synthase alpha is associated with stimulated acid secretion by gastric glands, Seventh international symposium on mass spectrometry in the health and life sciences, San Francisco, CA. Molecular & cellular proteomics. 4(8)(Suppl. 2): S472.
9. S. Karvar, L. Zhu, W. Wong, J. M. Crothers, and J. G. Forte, May 2005, Differencial distribution dynamics of syntaxin-1 and syntaxin-3 in gastric parietal cells, 106^th annual meeting of the American gastroenterological association, Chicago, IL. Gastroenterology. 128(4)(Suppl. 2):A-589. Abstract#: W1319.
10. R. Zhou, A. Kodani, V. Li, L. Zhu, J. Forte, December 2004, Phosphorylation of threonine-567 is not a functionally important site for apical membrane recruitment in parietal cells, 44^th Annual meeting of The American Society for Cell Biology, Washington D.C. Molecular biology of the cell. 15:171a, abstract # 950.
11. L. Zhu, Y. Liu, J. G. Forte, December 2004, Ezrin oligomers are the membrane-bound dormant form in gastric parietal cells, 44^th Annual meeting of The American Society for Cell Biology, Washington D.C. Molecular biology of the cell. 15:171a, abstract # 948.

Teaching experience: Mentoring undergraduate research

1. Lily Zhong, 2008-2009, Ezrin tyrosine phosphorylation in the gastric parietal cells.
2. Hai Van Le, 2008-2009, Functional proteomic studies on gastric parietal cells.
3. Cody Ender, 2008-2009, Identification of the activation factor for HK-ATPase.
4. Michael Starr, 2008, (summer student from Harvey Mudd College, CA) EGF effect on ezrin tyrosine phosphorylation and gastric acid secretion.
5. Bing Zhang, 2007-2009, Different expression pattern of ERM proteins in gastric gland.
6. Joy Makdisi, 2007-2008, Identification of apical marker proteins in gastric parietal cells.
7. Cheng Chen, 2007-2008, High turn-over of ezrin phosphorylation on T567.
8. Jason Hatakeyama, 2007-2008, Identification of apical marker proteins in gastric parietal cells.
9. Aditi Shastri, 2007-2008, Phosphorylation of ezrin in kidney proximal tubules: localization and activity.
10. Kevin Poon, 2007-2008, Two dimensional electrophoresis of ezrin: comparative study between gastric glands and kidney proximal tubules.
11. Aennes Abbas, 2006-2007, Expression of ezrin N-terminus in gastric parietal cell.
12. Tim Wu, 2006-2007, Ezrin and NHERF1 in gastric glands.
13. Joseph Delaney, 2005-2007, Ezrin binding proteins in gastric glands.
14. Sajan Patel, 2005-2006, Two-dimensional electrophoresis of ezrin from gastric glands: protein phosphorylation upon stimulation of gastric glands.
15. Veronica Lao, 2004-2005, Purification of ezrin from gastric glands.
16. Vincent Li, 2003-2004, Construction and characterization of recombinant adenoviruses expressing ezrin.
17. Ye Ye, 2000-2001, Development of a DNA vaccine against hepatitis C virus.

Teaching experience: Mentoring MD fellows

1. Raza Patel, 2009-, Development of an animal model for non-alcoholic steatohepatitis and the use thereof.

Teaching publication

1. Lixin Zhu, 2008, Teaching glycoprotein with a classical paper: knowledge and methods in the course of an exciting discovery ---- The story of discovering HK-ATPase -subunit. Biochemistry and Molecular Biology Education. 36:336-340.