Α-syn

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α-synuclein

 

 

α-synuclein

Protein Concentration: 20μM

Protocol:

  • Incubate α-syn in the differential media at a concentration of 200 μM for 24 h prior to adding to the cells. The protein is very soluble at this concentration.
  • After 24h, add 10 μl of this solution to the cells.
  • Incubate for 24h and do the MTT assay (48h for LDH assay)
  • If you want to do dose dependence with a drug, prepare the dilutions of the drug in the media in 70 μl volume (for 6 replicates). Add 70 μl of 200 μM α-syn to the drug solution. Take out 10 μl of media from the plated cells and add 20 μl of the peptide+ drug to the cells (6 replicates). Do MTT/LDH as required.
  • The viability of  20μM α-syn is ~60-70% by MTT

 

 

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