20.109(F07): M13.1 and MDS titers

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20.109(F07): Laboratory Fundamentals of Biological Engineering

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All data on this page was collected by the MIT's Biological Engineering undergraduate students in the Fall 2007 as part of a laboratory class, 20.109. It represents the culmination of an ambitious phage redesign project that began at the start of the term. Redesigned phage were tested for phage titers on a "complete" E. coli genome (MG1655), and on the indicated MDS, described in [1]

Contents

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6
10-8
10-10

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

T/R lab

Green Team

  • Team members: Arjun Naskar, Patrick Alayon
  • Description of M13.1 phage: p3F17Ag
  • Description of MDS strain: MDS60
  • Any notes about titers presented here: did not do a no phage control, M13K07 not grown at same time as other, some TA unevenly spread so uncertain if plaques could be seen.

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6 ~1000 ND 0(uneven) 0
10-8 0 (uneven) ND 0 0
10-10 0 ND 0 0

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

Purple Team

  • Team members:Phillip Samayoa, Justin Tan
  • Description of M13.1 phage: p7p9 unstuff
  • Description of MDS strain: MDS41
  • Any notes about titers presented here: no phage control has plaques

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6 ~500 ND 20 5
10-8 20 ND 3 5
10-10 ~100 ND 0 5

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

Red Team

  • Team members:Rosa Alvarez, Vivian Hernandez
  • Description of M13.1 phage:p9-YFP
  • Description of MDS strain:MDS42
  • Any notes about titers presented here:no phage control has no plaques, M13K07 not grown at same time as M13.1

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6 fully lysed ND 0 0
10-8 ~1000 ND 0 0
10-10 ~150 ND 0 0

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

Pink Team

  • Team members:Roshini Zachariah and Juliana Rotter
  • Description of M13.1 phage: p3F17Ag
  • Description of MDS strain: MDS60
  • Any notes about titers presented here:negative control had no plaques as expected

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6 ~100 ND 0 0
10-8 0 ND 0 0
10-10 0 ND 0 ND

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

Blue Team

  • Team members: Augusto Tentori and Jessica Keenan
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:M13K07 control not grown in parallel

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6 ~1000 ND ~1000 ~1500
10-8 16 ND 10 42
10-10

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

Yellow Team

  • Team members:Rebecca Kusko and Jessie Wang
  • Description of M13.1 phage:p8BAP
  • Description of MDS strain: MDS60
  • Any notes about titers presented here:

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6 ~100 ND 0 0
10-8 9 ND 0 0
10-10 0 ND 0 1

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml)

W/F lab

Green Team

  • Team members: Hao Ding and Neha Batra
  • Description of M13.1 phage: p8::BAP
  • Description of MDS strain: MDS12
  • Any notes about titers presented here: Did not get culture for MDS12xM13.1, ctrl showed no plaques (no contamination)

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS 12 phage = M13.1, strain = MG1655
10-6 >800 >800 14
10-8 74 63 1 (maybe)
10-10 1 (maybe) 0 0

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655
titer (PFU/ml) 7.6e11 PFU/ml 6.3e11 PFU/ml 1.4e9 PFU/ml

Purple Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Red Team

  • Team members: Scott Pollom and Spenser Skates
  • Description of M13.1 phage: dual p8
  • Description of MDS strain: MDS42
  • Any notes about titers presented here: Negative control had 160 plaques, so our data might be misleading. We used the 10^-8 dilution data to calculate the three PFU/ml values.

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
10-6 too many ND 800 too many
10-8 174 ND 113 225
10-10 350 ND 1 231

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS
titer (PFU/ml) 1.74e12 PFU/ml ND 1.13e12 PFU/ml 2.25e12 PFU/ml

Pink Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:

Plaque numbers

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS 43 phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS 43
10-6 2400 1632 17 27
10-8 78 43 68 25
10-10 2 2 31 49

Titer

phage = M13K07, strain = MG1655 phage = M13K07, strain = MDS43 phage = M13.1, strain = MG1655 phage = M13.1, strain = MDS43
titer (PFU/ml) 2.4E11 PFU/ml 1.63E11 PFU/ml 1.7E9 PFU/ml 2.7E9 PFU/ml

Blue Team

  • Team members: Matthew Loper and Kat Pak
  • Description of M13.1 phage:
  • Description of MDS strain: MDS43
  • Any notes about titers presented here: negative control showed 62 plaques so there was probably contaimination

Yellow Team

  • Team members:
  • Description of M13.1 phage:
  • Description of MDS strain:
  • Any notes about titers presented here:
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