20.109(S12):Post-selection IVT and journal club (Day6)

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20.109(S12): Laboratory Fundamentals of Biological Engineering

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RNA Engineering        Protein Engineering        Cell Engineering              

Contents

Introduction

You'll begin today by setting up your post-column IVT reactions. Then we will move to room 16-336 for the first round of journal club presentations.

Protocols

Part 1: IVT

Refer to the Day 3 protocol to prepare your IVT Master Mix, except this time shoot for a total volume of 40 μL rather than 80 μL per reaction. (Thus, you will add 6.6 μL of RT-PCR mixture to each reaction instead of 13.1 μL as you did before.) Note the time that you put your reactions on the 37 °C heat block.

Part 2: Journal club

We will move to 16-336 for the talks.

For next time

  1. Prepare the RT-PCR figure and associated text for the Results section.
  2. If you presented for journal club today, your first short reflective assignment is also due, both by email and as a hardcopy at the beginning of lab next time. Please remember to use the 20109.submit AT gmail DOT com address.
    • An awareness of your own strengths and weaknesses can often help you improve your future work. After you give your presentation, write a brief self-evaluation (200 words is plenty). Specifically, describe at least two things that you thought you did well, and at least two that could use improvement; a short paragraph will suffice for each of the two sections. Feel free to include both big-picture and detail-oriented comments.

Reagent list

Same as Day 3.
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