You'll begin today by setting up your post-column IVT reactions. Then we will move to room 16-336 for the first round of journal club presentations.
Part 1: IVT
Refer to the Day 3 protocol to prepare your IVT Master Mix, except this time shoot for a total volume of 40 μL rather than 80 μL per reaction. (Thus, you will add 6.6 μL of RT-PCR mixture to each reaction instead of 13.1 μL as you did before.) Note the time that you put your reactions on the 37 °C heat block.
Part 2: Journal club
We will move to 16-336 for the talks.
For next time
- Prepare the RT-PCR figure and associated text for the Results section.
- If you presented for journal club today, your first short reflective assignment is also due, both by email and as a hardcopy at the beginning of lab next time. Please remember to use the 20109.submit AT gmail DOT com address.
- An awareness of your own strengths and weaknesses can often help you improve your future work. After you give your presentation, write a brief self-evaluation (200 words is plenty). Specifically, describe at least two things that you thought you did well, and at least two that could use improvement; a short paragraph will suffice for each of the two sections. Feel free to include both big-picture and detail-oriented comments.
Same as Day 3.