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Gel Electrophoresis of PCR and Creating Lysis Buffer
Today, we ran our PCR products through a gel to determine if we successfully amplified our gene. Unfortunately, we failed to amplify our gene and we forgot to add the master mix to 2 of our 3 controls. There are many different things that could have made our PCR failed which include a failed DNA extraction, incorrect annealing temperatures, an incorrect master mix, etc. So we elected to try a different extraction protocol that we acquired from Dr. Spradling. In preparation for Thursday's lab, we created our lysis buffer.