840:153g:Projects/project2/2008/12/02

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The Sweet Smell of.....E.coli?

Today we had a long discussion on how to test if our part is functioning and also characterizing the part. We have proposed to exposure E.coli at log phase with UV irradiation in a deep petri dish for 1min, 2min and 10 mins. Before exposing the cells to UV, the intial pH and smell would be recorded. Each of the exposed plates would be checked for change of pH and wintergreen smell at 30min, 1 hour, 2 hour and 3 hour after the UV exposure. After the 1 hour check, the cells would be exposed again with UV according to their initial exposure time. Three replication for each exposure time would be done. This whole experiment would be repeated using fluorescent light instead of UV. Corey would be growing overnight cultures that would be used on Thursday to do the characterization experiment. Besides planning for this, we also pipeted 20uL of plasmids 1 and 4 from the miniprep in 1.5mL micro centrifuge tubes. 50uL of forward VF2 and reverse primer VR was given to Dr. Axel for sequencing.


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