840:153g:Projects/project4/2009/02/12

MoldBusters' Journal

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Thursday 2/12 Josh and Casy Came in early to start Agarose gel. Followed protocol discussed Tuesday. Prepared the part pSB1A2 + BBa_R0080 as digested DNA and undigested DNA for running on the electrophoresis gel. Digested DNA with the enzymes Xba1 and Spe1. Ran the gel for 30 minutes. The gel showed that the digested samples formed two fragments, the smaller of which was about 200 base pairs. This confirmed that the desired part has been isolated. Discussed preparing the plasmid for the incorporation of the aprE gene. Oggie, Derek, and Katy Oggie and Derek came in early to make Agarose gel. We checked PCR product using gel electrophoresis. Our + control worked but our DNA did not work. On Tuesday we will be calibrating pipets and redoing our DNA extraction.

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