840:153g:Projects/project4/2009/02/17

MoldBusters' Journal

Main project page Previous entry      Next entry

Tuesday 2/17 Josh and Casy The plasmid pSB1A2+R0080 samples were combined and digested with Pst1 and Spe1. The samples were purified by running the DNA through a column. Verification that the digestion was a success will be done on Thursday by running a gel. The digested plasmid was stored in the -20°C freezer, and should be ready for the aprE ligation. Oggie and Katy Did research on possibility of genetic differences between globiformis & niger strains, and strain 168 (we used DNA from strain 168 as the template when developing our primers) Did not find any results in regards to the two strains we are using Found new DNA extraction protocol at Bio.net Made TES buffer needed for new DNA extraction method