AG Daniel:Freezer
From OpenWetWare
Jump to navigationJump to search
AG Daniel
|
Antibodies• 4°C Antibodies |
Technical• Tools |
Organisation |
Contact UsForschungshaus Charité |
General Freezer (-20 °C)
Name | Cat. number | Company | Comment |
---|---|---|---|
Puromycin | |||
Zeocin | |||
M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant | Promega | ||
Bioline Bioscript | Bioline | ||
Bioline Random Hexamer Primers | Bioline | ||
Bioline RNase Inhibitors | Bioline | ||
Bioline 5x Reaction Buffer for Bioscript | Bioline | ||
Biotinylated Protein Ladder Detection Pack | Cell Signaling | ||
Proteinase K | |||
Monoclonal Anti-Phosphoserine Clone BR-45 | Sigma | ||
InSolution ALLN | |||
Alsterpaullone | Calbiochem | ||
Caspase Inhibitor 1 | Calbiochem | ||
Q-VD-oPh, Non-o-methylated | Calbiochem | ||
CdK1 Inhibitor CGP74514A | Calbiochem | ||
X-Gal | Promega |
Enzyme Freezer (-20 °C)
I-SceI Meganuclease kit
Klenow fragment
Polymerase
- GoTaq® Polymerase
- InviTaq Polymerase
- DreamTaq™ Green DNA Polymerase
10x DreamTaq™ Green Buffer 5 µl dNTP Mix, 2mM each 5 µl (0.2 mM of each) Forward primer 0.1 - 1.0 µM Reverse primer 0.1 - 1.0 µM Template DNA 10 pg - 1 µg DreamTaq™ DNA Polymerase 1.25 u Water, nuclease-free to 50µl Total Volume 50µl
Shrimp Alkaline Phosphatase
Phosphatase catalyzes the removal of 5´ phosphate groups from DNA and RNA. Since phosphatase-treated fragments lack the 5´ phosphoryl termini required by ligases, they cannot self-ligate. This property can be used to decrease the vector background in cloning strategies.
T4 DNA Ligase
TOPO® TA cloning kit
Restriction Enzymes
(status: 02/2010)
Name | Restriction site | NEBuffer 1 2 3 4 | Comments |
---|---|---|---|
AccI | 5'...GT MKAC...3'
|
050 050 010 100 | |
AfeI | 5'...AGC GCT...3'
|
025 050 025 100 | blunt |
AflII | 5'...C TTAAG...3'
|
050 100 025 100 + BSA | |
AluI | 5'...AG CT...3'
|
100 100 075 100 | blunt |
ApaI | 5'...GGGCC C...3'
|
025 050 000 100 + BSA | |
ApaLI | 5'...G TGCAC...3'
|
100 100 010 100 + BSA | |
AvaI | 5'...C YCGRG...3'
|
010 075 010 100 | |
AvaII | 5'...G GWCC...3'
|
050 075 010 100 | |
AvrII | 5'...C CTAGG...3'
|
100 100 050 100 | |
BamHI | 5'...G GATCC...3'
|
075 100 100 100 + BSA | |
BbsI | 5'...GAAGACNN NNNNNNNN...3' (N2)
|
100 100 025 075 | |
BclI | 5'...T GATCA...3'
|
050 100 100 075 [50°C] | |
BglII | 5'...A GATCT...3'
|
010 075 100 010 | |
BspEI | 5'...T CCGGA...3'
|
000 010 100 000 | |
BspHI | 5'...T CATGA...3'
|
010 100 050 100 | |
BstEII | 5'...G GTNACC...3'
|
050 075 100 075 + BSA [60°C] | |
BstXI | 5'...CCANNNNN NTGG...3'
|
025 100 100 050 [55°C] | |
Bsu36I | 5'...CC TNAGG...3'
|
000 025 100 000 + BSA | |
ClaI | 5'...AT CGAT...3'
|
010 050 050 100 + BSA | |
EcoNI | 5'...CCTNN NNNAGG...3'
|
100 100 075 100 | |
EcoRI | 5'...G AATTC...3'
|
100 100 100 100 | |
EcoRV | 5'...GAT ATC...3'
|
100 100 100 100 + BSA | blunt |
HaeII | 5'...RGCGC Y...3'
|
075 100 050 100 + BSA | |
HindIII | 5'...A AGCTT...3'
|
050 100 010 050 | |
HinfI | 5'...G ANTC...3'
|
075 100 075 075 | |
HpaI | 5'...GTT AAC...3'
|
025 050 010 100 | blunt |
I-SceI | 5'...TAGGGATAA CAGGGTAAT...3'
|
010 050 050 050 + BSA | |
KpnI | 5'...GGTAC C...3'
|
100 075 000 050 + BSA | |
MfeI | 5'...C AATTG...3'
|
075 050 010 100 | |
MluI | 5'...A CGCGT...3'
|
025 075 100 050 | |
NaeI | 5'...GCC GGC...3'
|
100 075 010 075 | blunt |
NcoI | 5'...C CATGG...3'
|
100 100 100 100 | |
NdeI | 5'...CA TATG...3'
|
075 100 075 100 | |
NheI | 5'...G CTAGC...3'
|
100 100 010 100 + BSA | |
NotI | 5'...GC GGCCGC...3'
|
000 050 100 025 + BSA | |
NruI | 5'...TCG CGA...3'
|
000 010 100 010 | blunt |
NsiI | 5'...ATGCA T...3'
|
010 075 100 025 | |
PacI | 5'...TTAAT TAA...3'
|
100 075 010 100 + BSA | |
PmlI | 5'...CAC GTG...3'
|
100 075 000 075 | blunt |
PshAI | 5'...GACNN NNGTC...3'
|
050 075 010 100 + BSA | blunt |
PstI | 5'...CTGCA G...3'
|
075 075 100 050 + BSA | |
PvuI | 5'...CGAT CG...3'
|
010 075 100 010 + BSA | |
PvuII | 5'...CAG CTG...3'
|
100 100 100 100 | blunt |
SacI | 5'...GAGCT C...3'
|
100 050 010 100 + BSA | |
SacII | 5'...CCGC GG...3'
|
025 075 010 100 | |
SalI | 5'...G TCGAC...3'
|
000 000 100 000 + BSA | |
SfiI | 5'...GGCCNNNN NGGCC...3'
|
000 100 010 100 + BSA [50°C] | |
SmaI | 5'...CCC GGG...3'
|
000 000 000 100 [25°C] | blunt |
SnaBI | 5'...TAC GTA...3'
|
025 050 025 100 + BSA | blunt |
SpeI | 5'...A CTAGT...3'
|
075 100 025 075 + BSA | |
SphI | 5'...GCATG C...3'
|
100 100 050 100 | |
SspI | 5'...AAT ATT...3'
|
050 100 050 050 | blunt |
StuI | 5'...AGG CCT...3'
|
100 100 050 100 | blunt |
SwaI | 5'...ATTT AAAT...3'
|
010 010 100 010 + BSA [25°C] | blunt |
Tth111 | 5'...GACN NNGTC...3'
|
100 025 025 100 [65°C] | |
XbaI | 5'...T CTAGA...3'
|
000 100 075 075 + BSA | DAM Methylierung!!! |
XcmI | 5'...CCANNNNN NNNNTGG...3'
|
010 100 050 050 | |
XhoI | 5'...C TCGAG...3'
|
075 100 100 100 + BSA | |
XmnI | 5'...GAANN NNTTC...3'
|
100 100 075 100 + BSA | blunt |
Name | Restriction site | NEBuffer 1 2 3 4 | Comments |
---|---|---|---|
AatII | 5'...GACGT C...3'
|
000 050 050 100 | Bernd |
AscI | 5'...GG CGCGCC...3'
|
000 010 010 100 | Bernd |
DraI | 5'...TTT AAA...3'
|
075 075 050 100 | Bernd |
EagI | 5'...C GGCCG...3'
|
010 025 100 010 | Bernd |
MspI | 5'...C CGG...3'
|
075 100 050 050 | Bernd |
Psp1406I / AclI | 5'...AA CGTT...3'
|
010 010 000 100 + BSA | Bernd |
SbfI | 5'...CCTGCA GG...3'
|
075 050 000 100 | Bernd |
SfcI | 5'...C TRYAG...3'
|
075 050 010 100 + BSA | Bernd |
SrfI | 5'...GCCC GGGC...3'
|
??? | Bernd |
SrfII | 5'...???...3'
|
??? | Bernd |
0402 Freezer (-20 °C)
Name | Cat. number | Company | comment |
---|---|---|---|
Brefeldin A | B-1009 | A.G. Scientific | |
GeneJET PCR Purification Kit | #K0701, #K0702 | Fermentas | |
Zeocin | Invitrogen | ||
Salubrinal | sc-202332 | Santa Cruz | |
GoTaq Polymerase | Promega |
Name | Restriction site | NEBuffer 1 2 3 4 | Comments |
---|---|---|---|
AflII | 5'...C TTAAG...3'
|
050 100 025 100 + BSA | |
BamHI | 5'...G GATCC...3'
|
075 100 100 100 + BSA | |
EcoRI | 5'...G AATTC...3'
|
100 100 100 100 | |
EcoRV | 5'...GAT ATC...3'
|
100 100 100 100 + BSA | blunt |
NdeI | 5'...CA TATG...3'
|
075 100 075 100 | |
NheI | 5'...G CTAGC...3'
|
100 100 010 100 + BSA | |
NotI | 5'...GC GGCCGC...3'
|
000 050 100 025 + BSA | |
NruI | 5'...TCG CGA...3'
|
000 010 100 010 | blunt |
PstI | 5'...CTGCA G...3'
|
075 075 100 050 + BSA | |
SacII | 5'...CCGC GG...3'
|
025 075 010 100 | |
SfiI | 5'...GGCCNNNN NGGCC...3'
|
000 100 010 100 + BSA [50°C] | |
SmaI | 5'...CCC GGG...3'
|
000 000 000 100 [25°C] | blunt |
StuI | 5'...AGG CCT...3'
|
100 100 050 100 | blunt |
XbaI | 5'...T CTAGA...3'
|
000 100 075 075 + BSA | DAM Methylierung!!! |
XhoI | 5'...C TCGAG...3'
|
075 100 100 100 + BSA |
S1 Freezer (-80 °C)
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
01 | ||||
02 | ||||
03 | ||||
04 | ||||
05 |
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
06 | Tim | Tim | Tim | Tim |
07 | Tim | Tim | Christian Herrberger | Christian Herrberger |
08 | Christian Herrberger | Christian Herrberger | Tim | Tim |
09 | Tim | Tim | Tim | Tim |
10 | Tim | Tim | Tim | Tim |
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
11 | ||||
12 | ||||
13 | ||||
14 | ||||
15 |
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
16 | ||||
17 | ||||
18 | ||||
19 | ||||
20 |
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
21 | ||||
22 | ||||
23 | ||||
24 | ||||
25 |
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
26 | ccDH5α, pUC19 DNA | ccDH5α STOCK | ||
27 | ||||
28 | ||||
29 | ||||
30 |
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
31 | ||||
32 | ||||
33 | ||||
34 | ||||
35 |
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
36 | ||||
37 | ||||
38 | ||||
39 | ||||
40 |
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
41 | ||||
42 | ||||
43 | ||||
44 | ||||
45 |
S1 Freezer #2 (-80 °C)
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
01 | ||||
02 | ||||
03 | ||||
04 |
S2 Freezer (-80 °C)
Status 01/2012
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
01 | ||||
02 | ||||
03 | ||||
04 |
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
05 | ||||
06 | ||||
07 | ||||
08 |
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
09 | ||||
10 | ||||
11 | ||||
12 |
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
13 | ||||
14 | ||||
15 | Jana; p73 Titer 2,5x105 | Jana; ΔNp73 Titer 6,5x104 | ||
16 | p53; Batch 52; Titer: 5x108 |
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
17 | ||||
18 | ||||
19 | ||||
20 |
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
21 | ||||
22 | ||||
23 | A&A; ARFtet Titer: 5x106 | A&A; ΔNp14ARFtet Titer: 1.5x107 | ||
24 |
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
25 | p14ARF; #8; 05/2005 | Annika & Philipp; LacZ; 06/05 | p14ARF 1+2 | p14ARF 1+2 |
26 | CEANBK | CEANBK #7.2 #7.7 | RSVβGAL / CMVβGAL | |
27 | Annika; p14ARF; 03.08.10; #25; Titer 2-3x107 | Annika; p14ARF; 23.03.07; Titer 6x108 | Annika; LacZ; 19.04.06; 1x108 | Annika; PUMA; 01.05.07; Titer x10? |
28 | Annika; p14ARF | Annika; p14ARF | p14ARF; 7/7I; 7/7II; 7/7III | p14ARF; 7/72; 04/08 |
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
29 | Tim; GFP; 11/08 | ?; LacZ; 04/07 | AHas; verschiedene BH3s | Tim; CHOPtet; Titer: 8,2x105 |
30 | ?; LacZ; T003; 03/06 | ?; NBKtet; 01/2007 | A&A; NBK | A&A; NBK; 03/06 |
31 | ?; verschiedenes | ?; ARF 03/07 | ?; ARF 01/2007 | ?; ΔNp14ARF; 03/06 |
32 | Tim; LacZ; 11/08; Titer: 1x108 | ?; Zellen | EMPTY | Tim ATF4tet; Titer 1,6x106 |
N2 tank (-196 °C)
Rack no. | Position 1 | Position 2 | Position 3 | Position 4 |
01 | ||||
02 | ||||
03 | ||||
04 |