AU Biomaterials Design Lab:Materials/pMAL-pIII
pMAL-pIII is a plasmid that contains a gene for the periplasmic expression of Maltose Binding Protein (MBP). This plasmid is sold as a control plasmid to ensure that a lab's expression system is working properly. We are using it for the expressed purpose of producing MBP. We are using MBP to template the growth of bioconjugated gold nanoparticles.
- Antibiotic: Ampcilin to 100μg/mL
- Medium: 10g tryptone, 5g yeast extract + 2g glucose/L
- IPTG stock (0.1M): 1.19g IPTG, 50mL water, filter to sterilize (cells are induced with 0.1mM IPTG)