Altman:Protocols/TC Protocols/Thawing ARPE-19

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Department of Physics, Willamette University

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Thawing ARPE-19 cells

Before starting:

  • Warm up Media+Serum in 37°C water-bath
  • Place a beaker for disposing of waste in the hood
  • Place a T-25 Flask in the hood
  • Place 9 mL of warmed Media+Serum into a 15 mL conical


1. Thaw aliquot of cells in gloved hands

2. Move cells into the 9 mL of media

3. Spin cells at 500 rpm for 3 minutes

4. Remove supernatant

5. Re-suspend the pellet in 5 mL of media

6. Place the cells into a T-25 flask
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