BCH072506-Construction of pSB3C6-J23030
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E [TT][Ptet][RBS] X [RFP] S P + [TT] E [Ptet] X [Lock3b] S P
Digest pJ23006-J23030 (EcoRI/SpeI, EcoRI, small-263bp)
Paste into pSB3C6-J23018 (EcoRI/XbaI, Neb2, large-2729bp)
Product is pSB3C6-J23030
