BCH081006-Construction of pJ23006-J23061 key/tRNA

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Overlap extension KB032/KB033 (141bp, XbaI/PstI)
Sub into pJ23006 (XbaI/PstI)
Product is pJ23006-J23061, screen with XhoI/AlwNI


KB032
cctagtctagagggagagatgccggagcggctgaacggaccgggatccacCTCGAGctaagcttcccg
KB033
ctgaactgcagcggccgctactagtatggcggagagagggggatttgaacccccggtagagttgcccctactccgggaagcttagCTCGAGg

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