BCH092406-Construction of pJ23006-J23085

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[tRNA key precursor J23061] + [B0015]
PCR ca998/G00101 on pSB1AK3-B0015 template (XbaI/PstI, neb3, 226bp)
Paste into pJ23006-J23061 (SpeI/PstI, neb2, large)
Product is pJ23006-J23085

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