BCH112106-construction of pJ23006-J23098, 99, 122, 123, 124, 125

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[j23085] + [BamHI/HindIII insert which changes key binding frame]
j23098

  • PCR Extend bh005F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
  • Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
  • Product is pJ23006-J23098
  • Screen by digesting product AlwNI/XhoI

j23099

  • PCR Extend bh006F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
  • Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
  • Product is pJ23006-J23099
  • Screen by digesting product AlwNI/XhoI

j23122

  • PCR Extend bh007F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
  • Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
  • Product is pJ23006-J23122
  • Screen by digesting product AlwNI/XhoI

j23123

  • PCR Extend bh008F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
  • Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
  • Product is pJ23006-J23123
  • Screen by digesting product AlwNI/XhoI

j23124

  • PCR Extend bh009F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
  • Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
  • Product is pJ23006-J23124
  • Screen by digesting product AlwNI/XhoI

j23125

  • PCR Extend bh010F/R. Digest (BamHI/HindIII, neb2, PCR cleanup)
  • Paste into pJ23006-J23085 (BamHI/HindIII, neb2, PCR cleanup)
  • Product is pJ23006-J23125
  • Screen by digesting product AlwNI/XhoI
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