The purpose of this experiment was to understand the fundamentals of using a bomb calorimeter, and measure a standard curve of temperature change of benzoic acid.
The materials of this experiment were as follows:
- Benzoic acid (premade pellets)
- Oxygen cylinder
- Isothermal bomb calorimeter
- 25°C water bath
- 2L volumetric flask
- 1mL pipet
We used the following procedure: "Bomb calorimetry: The energy concent of pizza" Stout, Roland P.; Nettleton, Faith E.; Price, Lynn M. J. Chem. Educ. 1985, 62, 438-439.
The raw data of our benzoic acid bomb cal.: Image:BOMB CALORIMETRY RAW DATA.xls
Standard Curve of Benzoic Acid Bomb Calorimetry
The purpose of this experiment was to learn some of the basics of polymerase chain reactions (PCR) and gel electrophoresis. This procedure simulates a crime scene investigation and allows us to analyze the genotypes of several DNA samples: one from a hypothetical crime scene and four from hypothetical suspects.
For a complete materials list refer to:
- Suspect DNA (A,B,C,D)
- Crime scene DNA
- Thermal cycler
The purpose of this procedure to use PCR to amplify DNA from a 'crime scene.'
Protocol is listed as in: "Crime Scene Investigator: PCR Basics™ Kit". Biotechnology Explorer™. Bio--Rad Laboratories, Inc.
Go to part 2 for continued protocol.
Go to part 2 for results.