BIOL368/F14:Class Journal Week 10

Chloe Jones

What was the purpose of these readings?
- The purpose of these readings was to get us more acquainted with DNA Microarrays and the way in which they work. The reading provided us with the necessary information in order to interpret a Microarray and be able to analyze based on color representation.
What did I learn from these readings?
- Before these reading I had no prior knowledge of microarrays so the reading was extremely helpful in providing some background information and allowing me to see the benefits of using them. I learned that color on a microarray corresponds to repression or a gene getting induced, and the overexpression and repression of genes could be monitored using microarrays. I also learned that microarrays are an extremely useful application and can aid in figuring out how gene coincide with diseases.
What did I not understand (yet) about the readings?
- I am still confused as to how you would observe if solely one gene affects the repression or overexpression of another gene. How would you tell that the way a gene is acting is the response to one gene in particular or a group of genes.

Chloe Jones 03:46, 15 October 2014 (EDT)Chloe Jones

What was the purpose of these readings?
- The purpose of these readings were to show the development and usefulness of DNA Microarrays in analyzing DNA expression patterns. The readings explained how to read a microarray and quantify the color based on the fold repressed/induced. They also applied the use of arrays in examining inhibition pathways.
What did I learn from these readings?
- I did not know much about DNA microarrays prior to this reading. I learned that each spot on the microarray represents segments of DNA amplified by PCR, and that the color is monitored over time. This array is monitored over time to see the progression of pathways by affecting transcription factors. I was also able to see how a variety of genes may be inhibited or induced, orchestrated together to support a final end goal depending on cell conditions (e.g. inducing the TCA cycle in the absence of glucose).
What did I not understand (yet) about the readings?
- I'm still confused on the process of calculating the fold factors, particularly for the repressed genes.

Isabel Gonzaga 00:56, 5 November 2014 (EST)

What was the purpose of these readings?
- The purpose of these readings was to provide an introduction to the basics and uses of DNA microarrays. They explained how they work, how the data that comes from them can be used and interpreted, and the practical applications they have in the world of genomics.
What did I learn from these readings?
- From the readings, I learned about what the many tiny, seemingly randomly colored dots in a DNA microarray slide ultimately represent. I learned how DNA microarrays are run and performed, and how the slides are interpreted. I also learned how DNA microarrays can be used to arrive at a conclusion about the expression of genes under particular circumstances.
What did I not understand (yet) about the readings?
- I did not fully understand the concept of hierarchical clustering discussed in the Campbell paper.