BIOL398-04/S15:Class Journal Week 5

From OpenWetWare

Jump to: navigation, search

Contents

William A. C. Gendron

  • Enzyme Reactions and Relation to Equation
  • What do yeast do in the lag phase of growth that is related to the reaction graph you drew?
    • The lag phase is a result of the yeast requiring time to convert the substrates to the desired products. The yeast will attempt to maintain homeostasis via these reactions and should tend toward this even if it is imperfect. This was seen in the research paper, even though it could have been shown better.
  • William A. C. Gendron 02:27, 17 February 2015 (EST):

Lauren M. Magee

  • Chemical Reaction Graph:
  • What do yeast do in the lag phase of growth that is related to the reaction graph you just drew?
    • During the lag phase of growth, yeast are readapting themselves to external factors. Under the proper conditions, with the presence of essential enzymes and appropriate levels of NH4+ and ATP, the reaction will eventually take place and the yeast will have surpassed the lag phase. The graphs provided in the attached file note the necessary items for a successful reaction to be carried out. Without any of these factors, a reaction will not occur and the yeast will remain in the lag phase.

Lauren M. Magee 01:15, 17 February 2015 (EST)

Lucia I. Ramirez

  • Click here to view the chemical reaction graph that defines the five enzyme reactions in yeast's nitrogen metabolism. A rate constant (kn) is included for each reaction arrow. The three product/substrate quantities are alpha-ketogluterate, glutamate, and glutamine.
  • What do yeast do in the lag phase of growth that is related to the reaction graph you just drew?
  • In the reaction graph, one can see that a product from one reaction becomes the substrate for the next reaction. For instance, glutamine can't be produced until enough glutamate is added to glutamine synthetase. In the same chain, glutamate can't be produced until enough concentration of alpha-ketoglutarate is added to NADPH-GDH. This sort of chain reaction that is seen in the graph could relate to yeast in their lag phase of growth, which is testing their environment before they react.

Lucia I. Ramirez 01:19, 17 February 2015 (EST)

Kristen M. Horstmann


Chemical Reactions Graph

  • During the lag phase, yeast cells are adapting and responding to the changing conditions. Different enzymes are being added and the concentrations are changing, so the yeast is utilizing this time to adapt to these changes and produce glutamate from the enzymes in order to grow and survive.
    Kristen M. Horstmann 21:59, 16 February 2015 (EST)

Alyssa N Gomes

  • Graph:

Part 1: Chemical Reactions Sketch Part 2: Chemical Reactions Sketch

    • This graph defines the five separate enzyme reactions in yeasts' nitrogen metabolism:
    • What do yeast do in the lag phase of growth that is related to the reaction graph you just drew?
      • During the lag phase of growth, we can see that yeast responds to these external and internal conditions, adapting and re-situating in order to continue to multiply cells. For every product that is made, it can be though of as the next new substrate for the next product to be made, so the yeast continuously prepares itself to strategically reproduce based on the previous transition of glutamine, glutarate and alpha-ketoglutarate.


Tessa A. Morris

  • Chemical Reactions
  • During the lag phase of growth, the yeast is adapting to the conditions under which they can achieve optimum growth. From the paper and class discussion it appears that in the lag phase, the cells are trying to generate a large amount of glutamate. The reactions that produce glutamate are the first three reactions written, utilizing the enzymes NADPH-GDH, GDA, and GOGAT to convert α-ketoglutarate and glutamine into glutamate (rate constants k1, k2, and k3).
  • Individual Journal Response

Tessa A. Morris 19:28, 16 February 2015 (EST)

Kara M Dismuke

  • Chemical Reactions
  • During the "lag phase," we observe the effects the changing conditions have on the yeast cells and their growth. Note, this takes into account the different rate constants for the different chemical reactions using the three product/substrate quantities (respectively).

--Kara M Dismuke 23:48, 16 February 2015 (EST)

Natalie Williams

The Chemical reactions

What do yeast do during the log phase of growth that is related to the reaction graph that was drawn?

  • It expresses how the cell is maturing and adapting to the conditions through synthesis of RNA and other molecules as they respond to the external and internal cellular environments.
Natalie Williams 00:46, 17 February 2015 (EST)

Karina Alvarez

Five Enzyme Reactions in Yeast's Nitrogen Metabolism Reactions Graph

What do yeast in the lag phase of growth that is related to the reaction graph above?

  • In the lag phase of growth, yeast cells respond to the changes in their environment by changing their internal environment. In this phase, they make different enzymes in different concentrations so that they can best adapt to their changing external conditions and optimize their growth.

See my individual journal here.

Personal tools