Biomod/2012/TeamSendaiA/Methods
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<!-- パンくずリスト --> <p id="Bread"> <a href="http://openwetware.org/wiki/Biomod/2012/Tohoku/Team_Sendai ">Team Sendai</a> > <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Top">Team Sendai A Top</a>> <a href=" http://openwetware.org/wiki/Biomod/2012/TeamSendaiA/Methodsa ">Methods</a> </p>
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<h2>AFM</h2> <img src="http://openwetware.org/images/7/76/SANY0194.JPG " alt="AFM" width="580" height="400"/> <p> <img src="http://openwetware.org/images/1/1b/SANY0195.JPG"width="200" height="180"/> AFM enables us to observe nano size structures composed of DNA. Make an observation sample. We put an observation object on a mica for five minutes and wash it in buffer. (Tris/Tris-HCL 20 mM Mg<sup>2+</sup> 12.5 mM pH = 7.4) </p> <h2> Electrophoresis</h2> <p><img src="http://openwetware.org/images/8/88/SANY0197.JPG" alt="AFM" width="580" height="400"/> We can see whether constructing DNA structures is achieved or not by Electrophoresis. It also informs us of DNA length of approximately.</br> The electrophoretic condition is cf. Result & Method.</font></p> <h2> How to create micelle</h2> <p><img src="http://openwetware.org/images/1/17/%E8%B6%85%E9%9F%B3%E6%B3%A2%E3%83%9B%E3%83%A2%E3%82%B8%E3%83%8A%E3%82%A4%E3%82%B6%E3%83%BC.jpg" alt="soni" "width="400" height="380"/> We dissolve lipid (OcTMAB) in chloroform. Take a small amount of solution, blow-dry it completely with a stream of argon gas. Add TAE Mg<sup>2+</sup> buffer or mQ and multiply the high frequency by ultrasonic liquid processor. </p>
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