BME100 f2013:W1200 Group14 L2

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BME 100 Fall 2013 Home
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Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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OUR TEAM

Name: Minh Pham
technician(s)
Name: Hannah Brutsche
Organization(s)
Name: Ashley Powell
Analyst(s)
Name: Vaasavi Sundar
Data/Stats(s)
Name: Brianna Schilling
Data/Stats(s)
Name: student
Role(s)

LAB 2 WRITE-UP

Descriptive Statistics

Experiment 1: Human

0mg:

    Average: 3.834
    Standard Deviation: 1.523010177
    Endpoint #	10
    Standard Error: 0.481618106

5mg:

    Average: 8.932
    Standard Deviation: 1.593931547
    Endpoint #: 10
    Standard Error: 0.504045412

10mg:

    Average: 61.622
    Standard Deviation: 30.11069386
    Endpoint #: 10
    Standard Error: 9.521837451

15mg:

    Average: 657.941
    Standard Deviation: 212.9429762
    Endpoint #: 10
    Standard Error: 67.33848166

Experiment 2: RAT

0mg:

    Average Inflammotin Levels: 10.516	
    Standard Deviation: 2.225551617
    Endpoint #: 5
    Standard Error: 0.445110323

10 mg:

    Average Inflammotin Levels:11.112
    Standard Deviation:7.402885924
    Endpoint#:5
    Standard Error: 1.480577185






[[]]==Results==

Experiment 1 human graph

Experiment 2:RAT Image.

(Please include well-labeled graphs of the results.)
link title




Analysis

Experiment 1:People
The average amount of inflammotin for people who were not injected was 3.834pg/mL. The average inflammotin levels for people injected with 5 mg was 8.932pg/mL. The average inflammotin levels for people injected with 10mg was 61.622pg/mL. The average levels for people injected with 15 mg was 657.941 pg/mL.
T-Test:

   0mg and 5 mg 	8.59631E-07
   0mg and 10 mg 	9.94377E-06
   0mg and 15 mg 	1.39436E-08
   5 and 10 mg         3.01859E-05
   5 and 15 mg 	1.57101E-08
   10mg and 15 mg 	6.4824E-08


Experiment 2:Rats
The difference in average inflammotin levels is .596 pg/mL between the rats who were not injected with the LPS and rats that were injected with 10mg of the LPS hormone. The average level of inflammotin for rats without LPS injections was 10.516 pg/mL and the average level for rats that were injected was 11.112 pg/mL. For the rats, the p-value is 0.8674.






Summary/Discussion

The higher doses of LPS, for example: 15mg, cause the protein inflammotin to increase in the blood stream of the humans by a factor of 171.6. The change in inflammotin levels from 10mg to 15mg was highly significant due to the increase of the LPS distributed. On experiment 1 on humans, the p-values between all the different doses were significantly less than 0.05 so all the differences were significant. For experiment 2, with rats, the p-value was 0.8674 so the difference in inflammotin levels was not significant. The lowest possible dose of LPS on humans is 5mg because the p-value between 0mg and 5mg was less than 0.05. This makes the 5mg dosage significant.