BME100 f2014:Group13 L6

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BME 100 Fall 2014 Home
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
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Name: Katherine Salazar
Name: Katherine Salazar
Name: Michael Nguyen
Name: Michael Nguyen
Name: Jacob Irwin
Name: Jacob Irwin
Name: Esteban Cruz
Name: Esteban Cruz
Name: Dalal Almuhaidib
Name: Dalal Almuhaidib
Name: Nicholas Olivar
Name: Nicholas Olivar


Bayesian Statistics

Overview of the Original Diagnosis System Image:Example.jpg

What Bayes Statistics Imply about This Diagnostic Approach

Calculations 1

Sensitivity to detect disease SNP.

Calculations 2

Specificity to detect disease SNP.

Calculations 3

Sensitivity to predict the disease.

Calculations 4

Specificity to predict the disease.

Computer-Aided Design


During the Computer-Aided Design Lab the TinkerCAD tool was used to assemble and modify the PCR machine. The TinkerCAD tool allowed for an adjustment of the size of the parts of the PCR Machine. TinkerCAD was also used to change the color and appearance of the PCR machine. The TinkerCAD tool provided a platform on which to upload pieces of the PCR machine and make various changes and revisions through manual adjustments using a set of basic functions.

Our Design


The biggest difference between the original PCR machine design and the new version is that the modified version is more compact and easier to transport. Another difference between the two is that within the machine more rows were added to fit an additional 8 PCR tubes. The extra space within the original PCR machine design has also been eliminated in order to allow for a more compact and streamlined design.

Feature 1: Consumables Kit

The PCR kit will be packaged in a way that all of the materials inside that are needed to perform the polymerase chain reaction will be promptly available in a more durable form. To compress this item into a smaller packaging, more rows were added to fit an additional 8 PCR tubes. Additionally, there will be an extra row of special tubes that will work to prevent fading of the SYBR I Green Dye by preventing entry of light into the tube. Also, the test tubes will be numbered so that each tube can be easily identified and so that there is no need to write on the tubes.

Feature 2: Hardware - PCR Machine & Fluorimeter

The main focus of our designed PCR machine set would be portability, meaning that it can be moved to places where it is needed. To do this, the PCR machine set must be durable so that any accidental drop or hit would not break it. Similar to the system used in the lab, our system would keep the PCR machine and Fluorimiter as separate machines, so that fluorimeter tests and PCR reactions can take place. The two devices would be a complete solid box that can attach to each other, with no parts sticking out of it that would potentially break off during when being boxed up and moved.

Other improvements over the current openPCR machine would be cutting down on the uncessessary space within the device. This would include putting the internal parts together in a way that less open space is between them, bringing the size down, making it more portable. In addition to this, the power supply would be moved outside of the device, cutting down the size of the device even further. The device would also be widened to allow for more test tubes slots, resulting in an increased rate of testing, as the time of reaction could not be increased.

Regarding the fluorimeter, the entire device would be one apparatus, with its own built in camera. While this may bring the overal cost of the device up, it would eliminate any possibility of unintentional user hardware error, such as changing resolutions. The device would be connected to the computer directly via USB, allowing for direct transfer to the computer rather than emailing it to one's self like it was used in the fluorimeter in the lab.

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