BME100 f2014:Group15 L2

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Contents

OUR TEAM

Name: Michael Catchings
Name: Michael Catchings
Name: Jessica Fong
Name: Jessica Fong
Name: Ben Heywood
Name: Ben Heywood
Name: Norihan Elsharawy
Name: Norihan Elsharawy
Name: Destiny Vidaure
Name: Destiny Vidaure
Name: Logan Migliorino
Name: Logan Migliorino

LAB 2 WRITE-UP

Descriptive Statistics


Experiment 1 - Rat Trial


Rat Trial Statistics
LPS Dose (mg)Average Inflammotin Measured (pg/ml)Standard DeviationStandard Error
0mg10.516 pg/ml2.2255516170.995296941
10mg11.112 pg/ml7.4028859243.310671231



Experiment 2 - Human Trial


Human Trial Statistics
LPS Dose (mg)Average Inflammotin Measured (pg/ml)Standard DeviationStandard Error
0 mg3.834 pg/ml1.5230101770.481618106
5 mg8.932 pg/ml1.5939315470.504045412
10 mg61.622 pg/ml30.110693869.521837451
15 mg657.941 pg/ml212.942976267.33848166



Results


Experiment 1 - Rat Trial

Rat Trial Results


Experiment 2 - Human Trial

Human Trial Results




Analysis


Experiment 1 - Rat Trial


t-Test: Two-Sample Assuming Equal Variances

Variable 1 Variable 2
Mean10.51611.112
Variance4.9530854.80272
Observations55
Pooled Variance29.8779
Hypothesized Mean Difference0
df8
t Stat-0.172401575
P(T<=t) one-tail0.433701749
t Critical one-tail1.859548038
P(T<=t) two-tail0.867403497
t Critical two-tail2.306004135



Experiment 2 - Human Trial


Anova: Single Factor

SUMMARY
Groups Count Sum Average Variance
Column 11038.343.8342.31956
Column 21089.328.9322.540617778
Column 310616.2261.622906.6538844
Column 4106579.41657.94145344.71112


ANOVA
Source of Variation SS df MS F P-value F crit
Between Groups3027016.69531009005.56587.253601951.40083E-162.866265551
Within Groups416306.02673611564.0563
Total3443322.72139



The p-value for the human study was 1.4e^-16. On the other hand, the rat study produced p-values of .433702 for the first set of data, and a value of .867403 for the second set of data. To put these numbers in context, the human p-value was substantially lower than the ideal p-value of .05, which concludes the test was extremely accurate. For the rat test, the p-value was , in both cases, higher than the ideal value of .05, which shows that this portion of the experiment was inconclusive.



Summary/Discussion


Based on the information gathered in the study, the averages, standard deviation, standard error, counts, and p-values were calculated. For the human trial, a single ANOVA was used to compare four sets of data, and in the rat trail a t-Test was used to compared two sets of data. With the collected data, a graph displaying the average value of inflammotin at each dosage of LPS (0mg, 5mg, 10mg, 15mg), verses the standard deviation was created. The graph of the humans showed that at LPS levels of 0mg, 5mg, and 10mg, the level of inflammotin stayed consistently under 100 pg/ml. Once the dosage was increased to 15mg, the inflammotin was increased to 650pg/ml. In the rat study, the level of inflammotin stayed relatively constant in both groups (0mg, and 10mg). The human study produced better results due to the amount and accuracy of the data. On the contrary, the rat data was inconclusive due to the lack of different dosage trials and inconsistent data. With that being said there is a definite positive correlation between LPS and humans, but not the same for rats.


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