BME100 f2016:Group7 W8AM L5

BME 100 Fall 2016	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Janai Crisostomo Name:Keon Okyere Name: Lindsay Lee Name: Edward Pomeroy Name: student Name: student LAB 5 WRITE-UP PCR Reaction Report Fluorimeter Procedure Imaging set-up Use iphone camera to record pictures of the solution drops Turn off the flash Set ISO to 800 or higher Set white balance to auto Set exposure to highest setting Set saturation to the highest setting Set contrast to the lowest setting Set the camera timer to 3 seconds Placing Samples onto the Fluorimeter Place 160 microliter drops of H2O betwwen the center rows on a glass test sheet using the pipettor Switch on the light in the excitation light Turn on and set up camera with correct settings Place camera in the cradle and line up the camera to take a profile picture of the solution drop Place 80 microliters of SYBR Green I to the solution drop Use your timer to take a picture of the solution drop and place the box over the apparatus to prevent light from shining in" repeat steps 1-6 for each solution and PCR sample record the data Data Collection and Analysis Images of High, Low, and Zero Calf Thymus DNA High http://i67.tinypic.com/11j0sy1.jpg Low http://i66.tinypic.com/2vsfd3p.jpg Zero http://i66.tinypic.com/10cw5r9.jpg Calibrator Mean Values http://i68.tinypic.com/14jamnc.jpg Calibration curves http://i64.tinypic.com/p2cg4.jpg http://i63.tinypic.com/24qmsmp.jpg Images of Our PCR Negative and Positive Controls Positive Control http://i68.tinypic.com/2q9xmiw.jpg Negative Control http://i64.tinypic.com/23r7cdh.jpg PCR Results: PCR concentrations solved http://i67.tinypic.com/a108p4.jpg http://i68.tinypic.com/fedwyx.jpg PCR Results: Summary Our positive control PCR result was 422.3 μg/mL Our negative control PCR result was 185.2 μg/mL Observed results Patient 57027 : The green was very visible on all 3 trials of this patient. We observed 402.1 ug/mL for this patient. Patient 97392 :The green dot was not visible in these trials and we observed 223.5 ug/mL for this patient. Conclusions Patient 57027 : This patient's results were very similar to the positive control so we concluded this patient was positive. Patient 97392 : This patient's results were very similar to the negative control so we concluded this patient was negative.