BME100 f2016:Group7 W8AM L5

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Contents

OUR TEAM

Name: Janai Crisostomo
Name: Janai Crisostomo
Name:Keon Okyere
Name:Keon Okyere
Name: Lindsay Lee
Name: Lindsay Lee
Name: Edward Pomeroy
Name: Edward Pomeroy
Name: student
Name: student
Name: student
Name: student


LAB 5 WRITE-UP

PCR Reaction Report

Fluorimeter Procedure

Imaging set-up
Use iphone camera to record pictures of the solution drops Turn off the flash
Set ISO to 800 or higher
Set white balance to auto
Set exposure to highest setting
Set saturation to the highest setting
Set contrast to the lowest setting
Set the camera timer to 3 seconds



Placing Samples onto the Fluorimeter

  1. Place 160 microliter drops of H2O betwwen the center rows on a glass test sheet using the pipettor
  2. Switch on the light in the excitation light
  3. Turn on and set up camera with correct settings
  4. Place camera in the cradle and line up the camera to take a profile picture of the solution drop
  5. Place 80 microliters of SYBR Green I to the solution drop
  6. Use your timer to take a picture of the solution drop and place the box over the apparatus to prevent light from shining in"
  7. repeat steps 1-6 for each solution and PCR sample
  8. record the data


Data Collection and Analysis

Images of High, Low, and Zero Calf Thymus DNA

High 11j0sy1.jpg

Low 2vsfd3p.jpg

Zero 10cw5r9.jpg

Calibrator Mean Values


14jamnc.jpg


Calibration curves

p2cg4.jpg


24qmsmp.jpg


Images of Our PCR Negative and Positive Controls

Positive Control 2q9xmiw.jpg


Negative Control 23r7cdh.jpg


PCR Results: PCR concentrations solved

a108p4.jpg

fedwyx.jpg


PCR Results: Summary

  • Our positive control PCR result was 422.3 μg/mL
  • Our negative control PCR result was 185.2 μg/mL


Observed results

  • Patient 57027 : The green was very visible on all 3 trials of this patient. We observed 402.1 ug/mL for this patient.
  • Patient 97392 :The green dot was not visible in these trials and we observed 223.5 ug/mL for this patient.


Conclusions

  • Patient 57027 : This patient's results were very similar to the positive control so we concluded this patient was positive.
  • Patient 97392 : This patient's results were very similar to the negative control so we concluded this patient was negative.



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