BME100 f2016:Group9 W8AM L6
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LAB 6 WRITE-UP
Overview of the Original Diagnosis System As a group we all took different samples of the PCR. Once we had taken all of the data, we used the program ImageJ to collect all of the data. Once every group collected their data from ImageJ, each group sent their data to be summarized with every other group. The challenges that could have affected the data of our lab are, the fact that our calibration could have been off when we swapped every glass slide. Another reason that our data could have been off is the fact that the camera was moved unintentionally when we switched the glass slides as well as when we added the drops our ovals took different shapes every time we drew them on image J. The effect of each drop being a different data point each time made it so that the data was faulty because it only relied on unreliable data. Once each group shared their data, the class was able to compare their data. The division of labor in this experiment was needed because one group could never expect to get so much data. Another error was the number of replicates per patient, more patients could have led to more reliable results. Overall, the results of the disease associated SNP was gained from every group's collective effort due to division of labor. The results yield was that the positive SNP had glowed and the negative SNP did not glow in ImageJ. The experiment was to test which drops of SNP glowed or did not glow.
Intro to Computer-Aided Design
In order to do any type of 3-D, we had to use a software that allowed us the create a 3-D shaped design for the new PCR machine. This required two websites in order to effectively model the PCR machine we wanted to improve. Using thingiverse, preloaded 3-D images were sent to tinkerCAD, CAD software, to actually model a new PCR design. No one in the group had prior experience working with these two programs. The tinkerCAD software made it so anyone could manipulate the models and change qualities such as the scaling and angles the parts needed to be assembled together. The Design lab with 3-D software was extremely helpful in modeling a new, innovative design for the PCR machine, already given the old parts to build off of.
The overall design of the PCR machine is that it is lighter weight and less bulky. This allows for easier transportation of the machine and decreases risk of damaging it. However, the innovative part of the new PCR machine is that has a solar panel attached to it and is equipped with a much more efficient battery. The attachment of a solar panel eliminates the need for it to be used only inside, now with the solar panel, groups such as Doctors Without Borders can use it in third world countries many of which do not have electricity.
Feature 1: Consumables
Since our new PCR machine utilizes solar panels, some essential consumables in our specific kit would includes things such as extra solar cells, batteries, and a device that can be wound up to make electricity given a situation such as cloudy or rainy weather. With the extra solar cells/panels, the pole that held the solar panel can hold more than just one panel, this would likely increase the longevity of the PCR machine because more solar panels and cells would mean more energy gained from the sun. The extra batteries would be for when a solar panel is not in use, a person could still use it and collect energy into a battery later to be put in the PCR machine. Lastly, given an bad weather situation, a crank-able device is included if the solar panels are not able to collect enough sunlight to convert to energy.
Feature 2: Hardware - PCR Machine & Fluorimeter
For our new PCR machine we will include both the Open PCR Machine and the fluorimeter to increase the flexibility of what the new PCR machine can do. Without these two essential pieces of hardware, then the PCR machine would not be able to get the wanted results and conduct the experiment. Given that the new PCR machine is smaller, the PCR machine itself and fluorimeter will be scaled to a smaller size to account for the new machine. The hardware will be easier to access and use because the fluorimeter will be built into the device to eliminate any steps outside the machine itself. The goal of the machine is to have it become an all-in-one PCR machine, this is better for the consumers because it will have the functions but take up less space.