BME100 f2016: Group2 W1030AM L4

BME 100 Fall 2016	Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: student Role(s) Name: student Role(s) Name: student Role(s) Name: student Role(s) Name: student Role(s) Name: student Role(s) LAB 4 WRITE-UP Protocol Materials Lab coat and disposable gloves PCR reaction mix, 8 tubes, 50 µL each: Mix contains Taq DNA polymerase, MgCl2, and dNTP’s DNA/ primer mix, 8 tubes, 50 µL each: Each mix contains a different template DNA. All tubes have the same forward primer and reverse primer A strip of empty PCR tubes Disposable pipette Cup for discarded tips Micropipettor OpenPCR machine PCR Reaction Sample List Tube Label PCR Reaction Sample Patient ID G2 + Positive control none *person tested positive for disease SNP G2 - Negative control none *person that does not carry the disease SNP G2 1-1 Patient 1, replicate 1 95353 G2 1-2 Patient 1, replicate 2 95353 G2 1-3 Patient 1, replicate 3 95353 G2 2-1 Patient 2, replicate 1 64816 G2 2-2 Patient 2, replicate 2 64816 G2 2-3 Patient 2, replicate 3 64816 DNA Sample Set-up Procedure Step 1 Step 2 Step 3... OpenPCR program Heated lid: 100°C Initial Research and Development PCR - The Underlying Technology SNP Information & Primer Design Background: About the Disease SNP Primer Design and Testing