BME100 s2014:T Group16 L5
BME 100 Spring 2014 | Home People Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3 Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||
OUR TEAM
LAB 5 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
The provided rack and an eraser was used to keep the phone in place. It is placed about 14cm from the fluorimeter which was raised by the height of two calculators to align the drop with the camera on the phone.
Solutions Used for Calibration
Placing Samples onto the Fluorimeter STEP 1: Set your micropipette to 80 microliters STEP 2: Add 80 microliters of SYBR Green 1 (with the concentration of 5 micrograms per microliters) with the micropipette onto the first middle two circles on the slide on the fluorimeter device. STEP 3: The drop should align to the smartphone camera and the led light. After setting the smartphone to the correct settings, use the timer and capture the picture. As the timer starts, place the box down and wait for it to capture the picture. STEP 4: Remove the box and the drop on the fluorimeter device. STEP 5: Save the picture into the computer (via email, Dropbox, etc.) and use Image J to find the RAWINTENDEN value. STEP 6: With Image J, draw an ellipse around the green version of the image. Measure the RAWINTDEN value. STEP 7: Repeat 1-7
Data AnalysisRepresentative Images of Samples WITH DNA
Image J Values for All Samples To save time on typing a new Wiki table from scratch, use THIS TOOL to auto-generate a Wiki table: Excel-to-Wiki Converter. Copy the headers and values from the Excel spreadsheet you made, paste them into the form field, click submit, copy the Wiki code that the tool generated, and replace TABLE GOES HERE (below) with your auto-generated code.]
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