# BME100 s2014:W Group14 L2

BME 100 Fall 2013 Home
People
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Photos
Wiki Editing Help

# OUR TEAM

 Name: Jose Duran Name: Shawn Garcia Name: Christian Keefer Name: Karthik Puncha Name: Austin Tielke

# LAB 2 WRITE-UP

## Descriptive Statistics

Experiment 1

Human Study

 LPS Dose Average Standard Deviation Endpoint Standard Error 0 mg 3.834 1.523010177 10 0.48161811 5 mg 8.932 1.593931547 10 0.50404541 10 mg 61.622 30.11069386 10 9.52183745 15 mg 657.941 212.9429762 10 67.3384817

Experiment 2

Rat Study

 LPS Dose Average Standard Deviation Endpoint Standard Error 0 mg 10.516 2.225551617 5 0.995296941 10 mg 11.112 7.402885924 5 3.310671231

Experiment 1

Experiment 2

## Analysis

Experiment 1

ANOVA Test: Single Factor
SUMMARY

 Groups Count Average Variance 0 mg 10 3.834 2.31956 5 mg 10 8.932 2.540618 10 mg 10 61.622 906.6539 15 mg 10 657.941 45344.71 Source of Variation SS dF MS F p-level F crit Between Groups 3027016.69 3 1009005.56 87.2536019 1.40E-16 2.86626555 Within Groups 416306.027 36 11564.0563 Total 3443322.72 39

 Post-hoc Tests t-test value Adjusted P-value to achieve significance Significant? 0mg vs. 5mg 8.59631E-07 0.008333333 Yes 0mg vs. 10mg 9.94377E-06 0.008333333 Yes 0mg vs. 15mg 1.39436E-08 0.008333333 Yes 5mg vs. 10mg 3.01859E-05 0.008333333 Yes 5mg vs. 15mg 1.57101E-08 0.008333333 Yes 10mg vs. 15mg 6.4824E-08 0.008333333 Yes

Experiment 2

 t-test value P-value to achieve significance Significant? 0.867403497 0.05 No

## Summary/Discussion

With regards to the first experiment, the results showed that an increased dosage of lipopolysaccharide (LPS) increased the levels of inflammotin proteins found in the body. The Bonferroni Correction indicated that the smallest dosage (5 mg) resulted in an increase in inflammotin protein levels which was proved by the significant difference between the 0 mg to 5 mg dosages. Although there was a high variance among the test subjects who took higher doses, each increasing dosage of LPS resulted in statistical significance.

Regarding the second experiment performed on rat subjects, 0mg and 10mg doses were given to a two groups of five rat subjects. It was found that there was no significant difference calculated upon the application of an unpaired t-test because the t-test value (0.86) was greater than the 0.05 p-value.

When analyzing the data between the two experiments, it can be stated that there is no significant impact of LPS on the rat subjects; however, there was a significant difference between the groups of the human subjects. It can be inferred that the human body is more sensitive to LPS than the body of a rat. Future experiments could be performed that involve a higher dosage of LPS given to rats if justification is needed to prove whether or not the body of a rat is significantly sensitive to the chemical compound lipopolysaccharide.