BME100 s2015:Group6 9amL1

From OpenWetWare
Jump to navigationJump to search
BME 100 Spring 2015 Home
People
Lab Write-Up 1 | Lab Write-Up 2 | Lab Write-Up 3
Lab Write-Up 4 | Lab Write-Up 5 | Lab Write-Up 6
Course Logistics For Instructors
Photos
Wiki Editing Help


OUR TEAM

Name: Sara Belko
Name: Priscilla Delgado
Name: Francisco Campa
File:AlaaImam.jpg
Name: Alaa Imam
Name: Luc Tieu

LAB 1 WRITE-UP

Independent and Dependent Variables

Independent: dosage (milligrams) of an inflammation inducing agent (lipopolysaccharide)

Dependent: amount of inflammatory protein in blood (Inflammotin)

Experimental Design

Groups

Five Groups

1) Control (0 mg placebo sugar pill)

2) 3 mg of lipopolysaccharide

3) 5 mg of lipopolysaccharide

4) 7 mg of lipopolysaccharide

5) 10 mg of lipopolysaccharide

(All groups take the pill daily before breakfast)


Age

"Elderly" = Ages 65 to 85


Number of subjects per group

10 people per group, 50 total




Subject Selection

Requirements:

1) Signs of inflammation

2) Must have inflammatory problems for at least 6 months

3) Should be off of other medicine 2 weeks prior to testing

4) Must be between the ages of 65 to 85

5) Males only

6) Moderately healthy lifestyle

7) Cannot have an allergic reaction to any drugs



Sources of Error and Bias

1) Removed gender

  - eliminated differences in Inflammotin level between gender

2) Human error (skipping doses)

  - results in a lower Inflammotin level

3) Diet

  - can cause additional inflammation

4) Other diseases

  - can weaken the immune system, other medication could interfere

5) Environment (weather, humidity, etc.)

  - dry vs. humid could increase inflammation

6) Duration of inflammation before treatment

  - may decrease the effectiveness of the lipopolysaccharide

7) Severity of inflammation before treatment

  - may decrease the effectiveness of the lipopolysaccharide