BME100 s2015:Projects5

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Lab 5: “Fluorescence Detection of DNA”

  • Content from PCR Labs B, C, D
    • Procedure
    • Data Analysis
    • SNP information & primer design

HW: Lab Report 5 (due 4/07/15 at 11:59 pm through OpenWetWare)

Grading Scheme:
10 points – Overall layout: Our Team section, grammar, style
15 points – Lab B/C content - Procedure: camera settings, calibration & solutions used for calibration, placing samples onto the fluorimeter
15 points – Lab B/C content - Data Analysis: representative images of samples, Image J values for all samples, fitting a straight line, PCR results summary
10 points - Lab D content - SNP information and primer design
50 points – Peer Assessment


OVERALL LAYOUTOUR TEAM section5 pts = Original & non-offensive images; real names3 pts = More than half original images & information1 pt = All default green & white avatars; default text
Grammar & style5 pts = Composition & layout perfect throughout3 pts = Few spelling errors; images too big or small1 pt = Many errors
PROCEDURECamera settings5 pts = Type of smartphone; settings where applicable, or "N/A"3 pt = Brand name only; vague1 pts = Missing all info
Calibration & solutions used5 pts = Clear description; distance btwn. camera & drop included; complete table in Wiki format3 pts = Missing one part of the information; Screen capture (e.g., jpeg) instead of wiki table2 pts = Missing two parts or poorly written
BONUS (5 points) - Photo of flourimeter set-up5 pts = Clear, good size; labels or caption added3 pts = Photo with no labels/ caption1 pt = Photo is over-sized; unclear
Placing samples onto the fluorimeter5 pts = Steps are thorough, clear, and easy to follow3 pts = Run-on paragraphs1 pt = Instructions are vague/ incomplete
DATA ANALYSISDrop images, INTDEN value table, plot with best fit line10 pts = Complete7 pts = Mising one part; very short overall4 pt = Missing two or more parts
PCR results summary5 pts = 100% Complete3 pts = More than 50% complete1 pt = Less than 50% complete
SNP INFO. & PRIMER DESIGNBackground5 pts = Well-written full sentences. 3 pts = Unclear, short (< 5 sentences), mostly phrases/ fragments 1 pt = Unclear, extremely short
Primer design & testing5 pts = Short summary & image of web tool results3 pts = Missing summary1 pt = Missing image or both

Note: *Empty/ plagiarized sections or borrowed images without references receive zero points

Important Information About Grading:

  • Plagiarism: Any images that are copy-pasted from the BME 100 workbook, from another group's Wiki page, or from the internet are considered as plaigiarism. Lab reports that contain any plagiarism will receive a zero and will be reported to James Collofello, Associate Dean for Academic and Student Affairs. Internet images (that are NOT from another group's or previous class's Wiki report) may be used as long as the source is properly cited.
  • Do not wait until the last minute to finish your report. Wiki pages are time-stamped after each "save". Only the version of your page that was saved by the deadline will be graded. Any changes made after the deadline will be ignored, no exceptions. Technical problems with the Wiki website must be reported at least 10 hours before the submission deadline.

Look for your Group's lab report link in the list of links on the right. If it is red instead of blue, do not click on it!

If the link is red, type the name of your Group's lab report link exactly as shown in the following text field and click the Create Page button.

This should open up an Edit page that is pre-populated with a lab report template code.

Group1_9amL5 Group2_9amL5 Group3_9amL5 Group4_9amL5 Group5_9amL5
Group6_9amL5 Group7_9amL5 Group8_9amL5 Group1_12pmL5 Group2_12pmL5
Group3_12pmL5 Group4_12pmL5 Group5_12pmL5 Group6_12pmL5 Group7_12pmL5
Group8_12pmL5 Group9_12pmL5 Group10_12pmL5 Group11_12pmL5 Group12_12pmL5
Group13_12pmL5 Group14_12pmL5 Group15_12pmL5 Group16_12pmL5 Group17_12pmL5

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