BXMU-PROJECT

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Result

1.Construction of BL21(DE3)pET28a-bis-His tag GDH-NOX

2. Expression and purification of bis-His tag GDH-NOX

Our final results are as following table:

3. Assembly of enzyme nanoring mediated by nickel ion

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4. Tanning experiment

4.1 DHA synthesis by purified enzyme
4.2 DHA synthesis by crude enzymee
4.3 Sunless Tanning

Methods

1 Genetic Manipulation

The method used to form the GUVs is “Vesicle Formation from emulsion”. This method consists in a controlled hydration of dried or nearly dried films of lipids deposited on a solid surface, in this case electrodes.

2. Gene expression and enzyme purification

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3. Nickel ion mediated enzyme assembly

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4. In situ synthesis of DHA and sunless tanning

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5. Characterization and analytic approaches

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